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Showing: 51 - 100 of 202 results

  1. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid
    #207757
    Purpose
    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207757-plasmid-map-sequence-id-415158

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  7. H2B-sfGFP-T2A-iCre

    Plasmid
    #178546
    Purpose
    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox and Mouse Targeting; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178546-plasmid-map-sequence-id-360157

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  10. SCN1Aprom(1-500)-H2B-GFP

    Plasmid
    #236162
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236162-plasmid-map-sequence-id-468412

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  11. EW410 IKZF1tar x8-H2B-GFP

    Plasmid
    #236156
    Purpose
    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with eight IKZF1 binding sit…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236156-plasmid-map-sequence-id-468408

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  13. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid
    #207759
    Purpose
    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207759-plasmid-map-sequence-id-415319

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  14. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

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  21. CMV-H2B-sfGFP-T2A-iCre

    Plasmid
    #183351
    Purpose
    Histone H2B-sfGFP and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183351-plasmid-map-sequence-id-360158

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  23. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  25. SCN1Aprom(335-406)-H2B-GFP

    Plasmid
    #236151
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236151-plasmid-map-sequence-id-468384

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  31. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid
    #102986
    Purpose
    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
    Depositor
    Marco Tripodi
    Article
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    102986-plasmid-map-sequence-id-200006

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  34. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE

    Plasmid
    #113843
    Purpose
    Histone H2B-superfolder GFP and tdTomato linked by T2A (V linker)
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113843-plasmid-map-sequence-id-223854

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  35. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE

    Plasmid
    #113842
    Purpose
    Histone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113842-plasmid-map-sequence-id-223654

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  36. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid
    #175571
    Purpose
    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175571-plasmid-map-sequence-id-344994

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  37. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  44. EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

    Plasmid
    #236148
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the UTRN promoter region
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236148-plasmid-map-sequence-id-468443

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Showing: 51 - 100 of 202 results