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Showing: 51 - 100 of 7088 results

  2. dSaCas9-KRAB

    Plasmid
    #205250
    Purpose
    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.
    Depositor
    Rasmus Bak
    Insert
    dSaCas9-KRAB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205250-plasmid-map-sequence-id-409107

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  3. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

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  4. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  5. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

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  6. pEC1.2/EC1.1_spCas9_GFP

    Plasmid
    #161933
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161933-plasmid-map-sequence-id-320699

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  7. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  8. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  9. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  10. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

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  11. pX551-CMV-CjCas9

    Plasmid
    #107035
    Purpose
    AAV plasmid expressing CjCas9 under control of CMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107035-plasmid-map-sequence-id-208555

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  12. pX551-miniCMV-CjCas9

    Plasmid
    #107033
    Purpose
    AAV plasmid expressing CjCas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    CjCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107033-plasmid-map-sequence-id-208551

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  13. pX551-miniCMV-SaCas9

    Plasmid
    #107032
    Purpose
    AAV plasmid expressing SaCas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107032-plasmid-map-sequence-id-208547

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  14. PB-iCas9

    Plasmid
    #160048
    Purpose
    inducible expression of Cas9
    Depositor
    Xiaojun Lian
    Insert
    Cas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    160048-plasmid-map-sequence-id-313847

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  15. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107024-plasmid-map-sequence-id-208524

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  16. pEC1.2/EC1.1_Cas9i_TagRFP

    Plasmid
    #227249
    Purpose
    CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pEC1.2/EC1.1
    Available since
    Nov. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    227249-plasmid-map-sequence-id-451525

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  17. Cas9-NAT

    Plasmid
    #64329
    Purpose
    Cas9 expression cassette carried by a yeast single-copy episome plasmid
    Depositor
    Yong-Su Jin
    Article
    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):
    Insert
    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64329-plasmid-map-sequence-id-227458

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  18. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  19. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  20. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  21. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  22. pUPD2 dCas9 (GB2664)

    Plasmid
    #160596
    Purpose
    CDS of dCas9 with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160596-plasmid-map-sequence-id-322070

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  23. pJAK184.tetR.PT5-3.dCas9

    Plasmid
    #232482
    Purpose
    optimized ahTet inducible dCas9 for insertion into C. difficile genome
    Depositor
    James Collins
    Article
    Collins et al Microbiology Resource Announcements
    Insert
    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetR-PT5-3
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232482-plasmid-map-sequence-id-461406

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  28. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  29. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196715-plasmid-map-sequence-id-389692

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  30. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140082-plasmid-map-sequence-id-292704

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  33. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Depositor
    Jan-Henning Klusmann
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181981-plasmid-map-sequence-id-366611

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  34. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  35. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  36. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  38. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid
    #206935
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206935-plasmid-map-sequence-id-407781

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  39. pAAV-CMV-Dpi2Cas9-puro

    Plasmid
    #232113
    Purpose
    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232113-plasmid-map-sequence-id-462529

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  40. pAAV-CMV-Slu1Cas9-puro

    Plasmid
    #232111
    Purpose
    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232111-plasmid-map-sequence-id-462528

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  41. pAAV-CMV-Slu2Cas9-puro

    Plasmid
    #232112
    Purpose
    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232112-plasmid-map-sequence-id-462527

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  42. pAAV-CMV-SeqCas9-puro

    Plasmid
    #232110
    Purpose
    Expresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232110-plasmid-map-sequence-id-462526

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  43. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid
    #127908
    Purpose
    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Human 5' HP1beta
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127908-plasmid-map-sequence-id-249780

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  44. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  45. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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  46. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104404-plasmid-map-sequence-id-204454

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  47. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  48. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

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  49. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  50. p-dCas9-HDAC1-Hygro

    Plasmid
    #104409
    Purpose
    transient expression of dCas9-HDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    HDAC1 (HDAC1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104409-plasmid-map-sequence-id-204471

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Showing: 51 - 100 of 7088 results