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Showing: 51 - 100 of 891 results

  7. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-2

    Plasmid
    #129054
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA2 (mouseTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129054-plasmid-map-sequence-id-256241

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  8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129042
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA2 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129042-plasmid-map-sequence-id-256275

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  9. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129030
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA2 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129030-plasmid-map-sequence-id-256140

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  11. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid
    #227608
    Purpose
    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    aa 2-83 deleted
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227608-plasmid-map-sequence-id-455573

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  12. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid
    #169191
    Purpose
    To express nsp10-nsp16 fusion protein in E. coli
    Depositor
    John Diffley
    Article
    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497
    Insert
    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
    Use
    Tags
    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…
    Expression
    Bacterial
    Mutation
    Codon optimised for E. coli
    Promoter
    T5
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169191-plasmid-map-sequence-id-333984

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  15. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Use
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

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  17. pLenti-EF1a-CD90.2-Rluc_miR

    Plasmid
    #163360
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    163360-plasmid-map-sequence-id-322108

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  18. pMX-mPGK-CD90.2-Rluc_miR

    Plasmid
    #163332
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mPGK
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163332-plasmid-map-sequence-id-322026

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  19. pMY-CD90.2-Rluc_miR

    Plasmid
    #163333
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163333-plasmid-map-sequence-id-322028

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  20. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

    Plasmid
    #163341
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163341-plasmid-map-sequence-id-322055

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  21. pMX-CAG-CD90.2-Rluc_miR

    Plasmid
    #163331
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163331-plasmid-map-sequence-id-322021

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  22. pMX-hFTH1-CD90.2-Rluc_miR

    Plasmid
    #163330
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human FTH1 promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hFTH1
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163330-plasmid-map-sequence-id-322015

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  23. pMX-hPGK-CD90.2-Rluc_miR

    Plasmid
    #163329
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human PGK promoter.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163329-plasmid-map-sequence-id-322013

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Showing: 51 - 100 of 891 results