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pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Expression

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pACSV-Ptc

Plasmid

#37675

Depositor

Philip Beachy

Article

Yao et al Cell. 2006 Apr 21;125(2):343-57.

Insert

ptc (ptc Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
GP735

Plasmid

#164537

Purpose

Expresses SmoM2-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

W539L

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP799

Plasmid

#164544

Purpose

Expresses Smo-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBS cPtc2 (CT#4)

Plasmid

#13942

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 2001 Nov 1. 239(1):15-29.

Insert

Ptc2 (PTCH2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
GP940

Plasmid

#164539

Purpose

Expresses SmoPi-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP938

Plasmid

#164538

Purpose

Expresses SmoPi-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PD19

Plasmid

#164528

Purpose

Expresses Flag-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

FLAG and Ub noK

Expression

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX mPtch-Cterm

Plasmid

#58704

Purpose

used for anti-mouse Patched antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1238-1413)

Promoter

tac

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMal mPtch-Cterm

Plasmid

#58705

Purpose

used for anti-mouse Patched antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1205-1434)

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
M7 pdnPKA-GFP

Plasmid

#16716

Depositor

Randall Moon

Article

Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

Insert

dnPKA (Prkar1a Mouse)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Dominant negative. Mutations at cAMP binding site…

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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GP777

Plasmid

#164533

Purpose

Expresses Smo noK-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Article

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Mutation

Promoter

ptc

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
GP821

Plasmid

#164552

Purpose

Expresses Smo-FLAG K434R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP758

Plasmid

#164541

Purpose

Expresses Sstr3-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP823

Plasmid

#164554

Purpose

Expresses Smo-FLAG K448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP822

Plasmid

#164553

Purpose

Expresses Smo-FLAG K444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP806

Plasmid

#164534

Purpose

Expresses Smo noK-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMuLE_ENTR_CBF-tdTomato_L3-L2

Plasmid

#113708

Purpose

Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

CBF (notch dependent promoter)

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFLmGFP-Gli2

Plasmid

#37672

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL3xHAmGli2

Plasmid

#37671

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PD29

Plasmid

#164542

Purpose

Expresses Drd1-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC3-C2cd3

Plasmid

#128871

Purpose

For mammalian expression of mouse C2cd3

Depositor

Aimin Liu

Article

Hoover et al Development. 2008 Dec;135(24):4049-58. d

Insert

C2cd3 (C2cd3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pBS cIhh (CT#431)

Plasmid

#13997

Depositor

Cliff Tabin

Article

Vortkamp et al Science. 1996 Aug 2. 273(5275):613-22.

Insert

Ihh (IHH Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
GP803

Plasmid

#164547

Purpose

Expresses Smo-FLAG K434, 444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

All three Lysines in cytoplasmic loop three of Sm…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP802

Plasmid

#164546

Purpose

Expresses Smo-FLAG K348, 360R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K348, 360R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Both the Lysines in cytoplasmic loop two of Smoo…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 YFP-Smo-Weyer's

Plasmid

#41014

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

EVC2 (aa 1177-1220) and YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS(A) cIhh (CT#432)

Plasmid

#13998

Depositor

Cliff Tabin

Article

Vortkamp et al Science. 1996 Aug 2. 273(5275):613-22.

Insert

Ihh (IHH Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc V5 dDispatched WT HA

Plasmid

#126405

Purpose

Expression of V5 dDispatched WT HA in Drosophila cells lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMuLE_ENTR_TOP-iRFP_L5-L4

Plasmid

#113866

Purpose

Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
iRFP713

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMuLE_ENTR_CBF-GLuc_L3-L2

Plasmid

#113868

Purpose

Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CBF (notch dependent promoter)
GLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ShhN-Ren

Plasmid

#37677

Depositor

Philip Beachy

Article

Ma et al Cell. 2002 Oct 4;111(1):63-75.

Insert

shh (Shh Mouse)

Use

Tags

renilla luciferase

Expression

Mammalian

Mutation

soluble form (see comments)

Promoter

CMV

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 41 - 80 of 222 results

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