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Showing: 41 - 80 of 149 results

  5. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  9. pSG5-Crkl mut SH3

    Plasmid
    #41705
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    W160K
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pSG5-Crkl mut SH2

    Plasmid
    #41703
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K
    Promoter
    SV40
    Available since
    April 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  13. pSG5-Crkl mut SH2/SH3

    Plasmid
    #41704
    Depositor
    Nora Heisterkamp
    Insert
    Crkl (CRKL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R39K and W160K
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  21. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. LT3GEPIR-CDK12shRNA

    Plasmid
    #220583
    Purpose
    To inducibly knockdown CDK12 expression
    Depositor
    Sean Lee
    Insert
    CDK12 shRNA (CDK12 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220583-plasmid-map-sequence-id-437726

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  23. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid
    #135281
    Purpose
    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-CDK12 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135281-plasmid-map-sequence-id-267208

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  24. p667-UBC-CDK12-V5_IDG-K

    Plasmid
    #135245
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135245-plasmid-map-sequence-id-268084

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  25. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid
    #135244
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-TurboID (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135244-plasmid-map-sequence-id-311575

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  26. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

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  31. Rac3 in pAcG2T

    Plasmid
    #38166
    Depositor
    Nora Heisterkamp
    Insert
    Rac3 (RAC3 Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  32. Rac1 in pAcG2T

    Plasmid
    #38164
    Depositor
    Nora Heisterkamp
    Insert
    Rac1 (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 16, 2012
    Availability
    Academic Institutions and Nonprofits only

  33. HA-tagged Abr

    Plasmid
    #38190
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  34. Abr in pAcG2T

    Plasmid
    #38162
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  35. HA-tagged Bcr

    Plasmid
    #38189
    Depositor
    Nora Heisterkamp
    Insert
    BCR (BCR Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
    38189-plasmid-map-sequence-id-453534

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  36. pSG5-Bcr

    Plasmid
    #39855
    Depositor
    Nora Heisterkamp
    Insert
    BCR (BCR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild type
    Promoter
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only

  37. Fer in pAcG2T

    Plasmid
    #38167
    Depositor
    Nora Heisterkamp
    Insert
    FER (FER Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  38. Bcr in pAcG2T

    Plasmid
    #38163
    Depositor
    Nora Heisterkamp
    Insert
    BCR (BCR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  39. Bcr/Abl P210 in pAcG2T

    Plasmid
    #38161
    Depositor
    Nora Heisterkamp
    Insert
    Bcr/Abl P210 (BCR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 80 of 149 results