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Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1
Plasmid#205447Purposehuman AAVS1-targeting plasmid for expression of mouse CR1DepositorInsertmCR1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188769PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188770PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP
Plasmid#188902PurposedCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9-KRAB(Kox1)-MeCP2
UseLentiviralTagsNLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…ExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188765PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188772PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterCMVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188774PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188771PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEFSAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188901PurposedCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertSID-dCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1
Plasmid#205444Purposehuman AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59DepositorInsertiCasp9-HLA-E SCT-hCD55
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1
Plasmid#205446Purposehuman AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55DepositorInsertHSV TK-H2-Kb SCT-mCD47-mCD55
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1
Plasmid#205445Purposehuman AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCTDepositorInsertiCasp9-Crry-mCD59-Qa1 SCT
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1
Plasmid#205443Purposehuman AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55DepositorInserthCD46-HLA-G SCT-hCD47-hCD59
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS
Plasmid#153416PurposeBackbone for cloning downstream of UCOE-SFFVDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
control (pGS701)
Plasmid#160037PurposepLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-J-TAG (pGS730)
Plasmid#160038PurposepLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsert3xHA-sfGFP-JPH-TMD
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-M-TAG (pGS555)
Plasmid#160040PurposepLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertMMGT1-mScarlet-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-P-TAG (pGS594)
Plasmid#160039PurposepLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertCP450TMD-APEX2-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH0004
Plasmid#174087PurposeUCOE-SFFV-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH0007
Plasmid#174162PurposeUCOE-EF1A-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUG-IRES-mCherry
Plasmid#222109PurposeStart codon reporter (WT AUG)DepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUA-IRES-mCherry
Plasmid#222116PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAUA start codonPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AGC-IRES-mCherry
Plasmid#222119PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAGC start codonPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CUG-IRES-mCherry
Plasmid#222110PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationCUG start codonPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ACG-IRES-mCherry
Plasmid#222113PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationACG start codonPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only