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Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CyTERM-mGFP
Plasmid#62237PurposeER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.DepositorInsertCyTERM-mGFP
UseTagsmGFPExpressionMammalianMutationsignal anchor of rabbit cytochrome p450 and monom…PromoterCMVAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-GFP
Plasmid#220198Purposemini-Tn7 GFP expression vector - LacI/pLac promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterLacI/pLacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mEGFP
Plasmid#168490PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mPAGFP
Plasmid#168493PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG42-GFP
Plasmid#220202Purposemini-Tn7 GFP expression vector - pBG42 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG35-GFP
Plasmid#220201Purposemini-Tn7 GFP expression vector - pBG35 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-GFP
Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP-mCherry
Plasmid#27763DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFP-monomeric Cherry (tandem)ExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertGja1 (Gja1 Rat)
UseTagsmonomerized superfolder GFPExpressionMammalianMutationPromotercmvAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx43K258stop-msfGFP
Plasmid#69023PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationdelete codons 258-381 and fuse in frame to monome…PromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx26-sfGFP
Plasmid#69026PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsnon-monomerized superfolder GFP (V206)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx30-msfGFP
Plasmid#69019PurposeExpresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromotercAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd-mEGFP
Plasmid#214369PurposeExpression of the monomeric Green Fluorescent ProteinDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1.1-Tr2-eGFP
Plasmid#162782PurposeFluorescent reporter for protein expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-Cα-mPAGFP
Plasmid#114131PurposeMammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertof the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1-gs4_EF1a-Cas9-2A-GFP
Plasmid#118414PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locusDepositorInsertgRNA AAVS1 (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV_mIFP IRES EGFP
Plasmid#72443PurposeExpressing mIFP and EGFP in mammalian cellsDepositorInsertmIFP T2A HO1 IRES EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-CαN-mPAGFP
Plasmid#114130PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only