-
Plasmid#155014PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cellsDepositorInsertiSpyMac
UseTagsExpressionMammalianMutationPID grafted onto SpyCas9 N-terminal domain, R221K…PromoterEF1ɑAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpyB
Plasmid#220186PurposesgRNA expression vector - pBG35 promoterDepositorInsertpBG35 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESF140_HisSpy_DDB1∆B
Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…PromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
UseTagsHis6 TagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15A_SpyCas9_CmR
Plasmid#119162PurposeExpresses S. pyogenes Cas9DepositorInsertCas9 from S. pyogenes (NEWENTRY )
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK+-hp
Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
p15A_J23108_SpydCas9_CmR
Plasmid#119163PurposeExpresses S. pyogenes dCas9DepositorInsertdCas9 from S. pyogenes
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only