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Plasmid#131505PurposemCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-inDepositorInsertmCherry_KASH
UseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-HygroR-T2A-TetOn3G
Plasmid#179887PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsHygromycin resistanceExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CSIV-TRE-ASCL1
Plasmid#154883PurposeDoxycycline-dependently expresses human ASCL1.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
Plasmid#187341PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertdCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
Plasmid#195542PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2521
Plasmid#142319PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAATF (AATF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1
Plasmid#169449Purposeexpression of TRF1-mcherrry and sgRNA targeting telomeresDepositorInsertmCherry-TRF1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2774
Plasmid#142160PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5C (KDM5C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK-hACE2
Plasmid#161612PurposeExpression of human ACE2 in mammalian cellsDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl3
Plasmid#86680PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorInsertATL3 (ATL3 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IKKbeta (S177E,S181E)-pcw107
Plasmid#64609Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertIKBKB
UseLentiviralTagsFLAGExpressionMutationS177E, S181EPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-METAP1-3xFLAG
Plasmid#163453Purposelentiviral overexpression vector for human METAP1DepositorInsertMETAP1 (METAP1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceMarch 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3386
Plasmid#144862PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS
Plasmid#199351PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFAExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag
Plasmid#132412Purposeoverexpression in human cellsDepositorInsertdAdmCas13b
UseLentiviralTagsflagExpressionMammalianMutationR269A; H274A; R837A; H842APromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only