Addgene

We narrowed to 15,711 results for: puromycin

Showing: 3961 - 3980 of 15711 results

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pQCXIP X2/pTER shLUC (w454-1)

Plasmid

#17491

Purpose

Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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shCDK6(2)

Plasmid

#73553

Purpose

shRNA(2) against CDK6

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
shCDK6(1)

Plasmid

#73552

Purpose

shRNA(1) against CDK6

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
CJ40 (let-7 seed mutant perfect site)

Plasmid

#17773

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 seed mutant perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pMA3200

Plasmid

#46855

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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AIRAP 7: AIRAPmyc.pBABEpuro

Plasmid

#21797

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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PRAS40 (mouse) shRNA

Plasmid

#15672

Depositor

John Lawrence

Article

Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pBABE puro rat IRS-3

Plasmid

#11362

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-3 (Irs3 Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-EZH2

Plasmid

#222528

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

EZH2 (EZH2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BRCA1

Plasmid

#222530

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BRCA1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB1

Plasmid

#222534

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB1 (HMGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRrsGreenF-E

Plasmid

#205764

Purpose

Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCEFL-EGFP-TEADiv2

Plasmid

#228872

Purpose

Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS927

Plasmid

#228238

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

Inserts

mCherry
Beta globin

Use

Tags

3xFLAG, HA, and PuroR

Expression

Mammalian

Mutation

Promoter

TRE3GV and none

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-Rab5

Plasmid

#228139

Purpose

Expresses GFP-Rab5 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab5 (Rab5a Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-24xMS2

Plasmid

#212629

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-mTagBFP2-Sec61B

Plasmid

#228136

Purpose

Expresses mTagBFP2-Sec61B in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Sec61B (SEC61B Human)

Use

Retroviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-SNAP-Omp25

Plasmid

#228137

Purpose

Expresses SNAP-Omp25 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

transmembrane region (C-terminus)

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-2xMS2

Plasmid

#212627

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-Puro-12xMS2

Plasmid

#212628

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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