Addgene

We narrowed to 18,124 results for: omp

Showing: 3961 - 3980 of 18124 results

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PT7lacO-RRF-His6

Plasmid

#224374

Purpose

Express RRF protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RRF (frr E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-IF3

Plasmid

#224368

Purpose

Express IF3 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

IF3 (infC E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-IF1

Plasmid

#224366

Purpose

Express IF1 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

IF1 (infA E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RF1-His6

Plasmid

#224372

Purpose

Express RF1 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

RF1 (prfA E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7

Plasmid

#220468

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7.M4

Plasmid

#220469

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 Full (1-261)

Plasmid

#99654

Purpose

Expresses dSp Cas9 fused to p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

p65 activation domain

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-STOP

Plasmid

#215840

Purpose

Expression of FLAG/HA tag

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

Plasmid

#210842

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bamh1

Plasmid

#203298

Purpose

For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bgl11

Plasmid

#203299

Purpose

For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Xba1

Plasmid

#203300

Purpose

For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Not1

Plasmid

#203301

Purpose

For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Kpn1

Plasmid

#203297

Purpose

For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABmNeonC

Plasmid

#178464

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmNeon, TatC

Use

Tags

mNeonGreen fused to TatB

Expression

Bacterial

Mutation

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABCmNeon

Plasmid

#178465

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatCmNeon

Use

Tags

mNeonGreen fused to TatC

Expression

Bacterial

Mutation

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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