-
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT
Plasmid#128551PurposeLentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND3
Plasmid#172623PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND2
Plasmid#172629PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM3
Plasmid#72210PurposeMammalian expression of aa 227-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-226PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM1
Plasmid#72208PurposeMammalian expression of aa 1-372 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 373-860PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM2
Plasmid#72209PurposeMammalian expression of aa 1-517 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 518-860PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM4
Plasmid#72211PurposeMammalian expression of aa 373-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-372PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM5
Plasmid#72212PurposeMammalian expression of aa 518-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-517PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
px459-AMBRA1 #3
Plasmid#172606PurposeExpresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-PuroDepositorInsertgRNA against AMBRA1 (AMBRA1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1(T286A)
Plasmid#172631PurposeExpresses untagged cyclin D1(T286A) in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTagsExpressionMammalianMutationThr286AlaPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CCR5 HHE Short
Plasmid#137697PurposeEncodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferaseDepositorInsertCCR5 Promoter (CCR5 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet-ECHB(35-475)
Plasmid#96890Purposeexpresses His-tagged mouse ECHB (35-475) in E.coli cellsDepositorInsertTrifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
UseTagsHis6 tagExpressionBacterialMutationdeleted amino acid 1-34PromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CCR5 HHG Short
Plasmid#137699PurposeEncodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferaseDepositorInsertCCR5 Promoter (CCR5 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763)-FLAG (no His-tag)
Plasmid#96887Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
UseTagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36PromoterT7Available sinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH752-CEN-RLuc/min103maxCFLuc
Plasmid#38218DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first 103 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH751-CEN-RLuc/min53maxCFLuc
Plasmid#38217DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first 53 codons of the original FLuc gene wer…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A PH+DH domains
Plasmid#69793PurposePebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH +PH domains (pbl Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationPbl-A (NP_729306.1) amino acids 385-647.Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP∆682-688-shTACC3
Plasmid#69116PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of amino acids 682-688 (region containin…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only