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Plasmid#162606PurposePre-express Cas9 in yeastDepositorInsertTef1-Cas9
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-3xHA-Puro
Plasmid#207729PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pADH110
Plasmid#90982PurposeNAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.DepositorInsertsNAT 2 of 2
pSNR52
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Blast
Plasmid#207710PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-moxGFP
Plasmid#207677PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-miniTurbo
Plasmid#207692PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ1389
Plasmid#215348PurposeNla CRISPRi Delivery VectorDepositorInsertNla CRISPRi Genes (Cas5, Cas8c, Cas7)
UseCRISPR; Nla i delivery vectorTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRU44
Plasmid#167666PurposeENTRY vector for gRNA4 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT43
Plasmid#204299PurposeFor introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-Zeo
Plasmid#158709PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…TagsExpressionBacterialMutationPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU42
Plasmid#167664PurposeENTRY vector for gRNA2 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-p65-HSF1-Blast
Plasmid#192652Purpose3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance markerDepositorInsertscFv-p65-HSF1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
vHB8
Plasmid#193454PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInsertGFP-Cas9-NLS
UseCRISPRTagsGFPExpressionMutationPromoterGAPDHAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-Hyg
Plasmid#158708PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…TagsExpressionBacterialMutationPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU43
Plasmid#167665PurposeENTRY vector for gRNA3 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330S-4
Plasmid#58780PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA2 TKIT
Plasmid#169442PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only