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Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA6-N-3XFLAG-Ubxd8
Plasmid#123599DepositorInsertFas associated factor family member 2 (Faf2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-ELP[V-30]
Plasmid#67014PurposeExpresses ELP[V-30] in E. coliDepositorInsertELP[V-30]
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Neo
Plasmid#60525PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)
Plasmid#140534PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2DepositorInsertOsTIR1(F74G) mAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-Keap1
Plasmid#28023DepositorInsertKeap1 (KEAP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pER-rxRFP1.4
Plasmid#67842Purposeredox-sensitive red fluorescent protein rxRFP1.4, endoplasmic reticulum localizedDepositorInsertrxRFP1.4
UseTagsER localization, ER retention, and His TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Tau-cYFP
Plasmid#92205PurposeRetroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)DepositorInsertMAPT (MAPT Human)
UseRetroviralTagsYFP(156-239)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Zeo DEST (644-1)
Plasmid#17294Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, ZeoDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pAV-U6+27-Tornado-Squash
Plasmid#177915PurposeExpresses Squash aptamer as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Squash
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNuc-rxRFP1.1
Plasmid#68131Purposeredox-sensitive red fluorescent protein rxRFP1.1, nuclear localizationDepositorInsertrxRFP1.1 and nuclear localization sequence
UseTagsHis Tag and nuclear localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-α-Syn-nYFP
Plasmid#92203PurposeRetroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)DepositorInsertSNCA (SNCA Human)
UseRetroviralTagsYFP(1-155)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
35S-eGFP-nosT
Plasmid#80127PurposeTransient expression vector for eGFP in plantsDepositorTypeEmpty backboneUseTagseGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CD81-10
Plasmid#54031PurposeLocalization: Tetraspanin/Membrane, Excitation: 487, Emission: 509DepositorInsertCD81 (CD81 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGCC4
Plasmid#37058DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only