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Double UP mNeon to mScarlet

Plasmid

#125134

Purpose

Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-UBCp-hMGT-Ef1a-puro

Plasmid

#172396

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx2

Plasmid

#183654

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBHRSF184

Plasmid

#89482

Purpose

Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Use

Tags

Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF0235

Plasmid

#142488

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYCN (MYCN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

mini-CAG

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Michael Davidson, Robert Campbell

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Use

Tags

mWasabi

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSmac-GFP

Plasmid

#40881

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac-GFP (DIABLO Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pCK122 pDESTattB_exorh:mCherry

Plasmid

#213511

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al bioRxiv 2023.12.08.570868

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK6

Plasmid

#23836

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK6 (MAPK6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEYFP-NR1a

Plasmid

#17928

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti 4.1 Ex miR200b-200a-429

Plasmid

#35533

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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pKC-Tol2

Plasmid

#85600

Purpose

Source of Tol2 transposase driven by a mini-CAGS promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

mini-CAG

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJA42

Plasmid

#59930

Purpose

gRNA for cleavage at rol-6(su1006) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rol-6(su1006) gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR2

Plasmid

#184047

Purpose

Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

ExRai-AktAR2

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Use

Tags

His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pN3-mTagBFP-pHluorin-CAAX

Plasmid

#178345

Purpose

Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

mTagBFP-pHluorin-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-Mart1-SABR

Plasmid

#119052

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

A0201-Mart1-SABR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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