We narrowed to 26,324 results for: sta

1. DN1-Gli3 in AINGpCAGGS

   Plasmid

   #121970

   Purpose

   medium Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-864)

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3019_pETcon-SARS-CoV-2-RBD_E484K

   Plasmid

   #184404

   Purpose

   yeast surface display of the SARS-CoV-2 Eta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   E484K

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ZKSCAN1_Tau7-12WT

   Plasmid

   #194166

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 7-12 WT
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_ZKSCAN1_Tau10-12WT

   Plasmid

   #194163

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
   

   Use

   Tags

   3X flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTER GAMMA-TUBULIN shRNA Human

   Plasmid

   #87955

   Purpose

   reduced the expression of gamma-tubulin in human cells

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   sh gamma-tubulin (TUBG1 Human)
   

   Use

   Tags

   No-tag

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene.…

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

   Plasmid

   #121448

   Purpose

   To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

   Depositor

   Brian Chen

   Article

   Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

   Insert

   RFPnols-PQR-ZeocinR (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN-A112C-GpA-G83I

   Plasmid

   #191239

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Use

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and Glycin…

   Promoter

   T7 promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

   Plasmid

   #184406

   Purpose

   yeast surface display of the SARS-CoV-2 Beta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   K417N+E484K+N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-B-838;+80pCalb2

    Plasmid

    #66750

    Purpose

    luciferase reporter for Calb2 promoter (-838 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-838bp +80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKIF1C-2xFLAG

    Plasmid

    #130979

    Purpose

    Expression of KIF1C-2xFLAG in mammalian cells.

    Depositor

    Anne Straube

    Article

    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

    Insert

    KIF1C-2xFLAG (KIF1C Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    4 silent mutations that make this construct resis…

    Promoter

    CMV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV Hygro DHFR.cHSF1

    Plasmid

    #134738

    Purpose

    Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202

    Promoter

    CMV

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmirGLO-Calb2-3'UTR

    Plasmid

    #68363

    Purpose

    Luciferase miRNA Target Expression Vector

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    Calb2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    C to T change in UTR

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pmirGlo-CALB2-3'UTR mir-30 mut2

    Plasmid

    #74429

    Purpose

    Calb2 UTR with mutated mir-30 site 1 and 2

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

    Insert

    CALB2 3'UTR (CALB2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)

    Plasmid

    #238189

    Purpose

    Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    PLAGL1 sgRNA CRISPRa (PLAGL1 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid

    #238169

    Purpose

    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    CD45 sgRNA CRISPRa (PTPRC Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R2pH-LAMP-FRB

    Plasmid

    #225138

    Purpose

    R2pH-LAMP1-labelled lysosomes with FRB

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid

    #202331

    Purpose

    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

    Depositor

    Didier Stainier

    Article

    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

    Inserts

    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    

    Use

    Tags

    base 238 C -> A mutation causing a PTC at codo…

    Expression

    Worm

    Mutation

    Promoter

    This is a promoter sequence for C. elegans eft-3,…

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNK7188

    Plasmid

    #219768

    Purpose

    Vector for expression Pv4CL1 in yeast (P.pastoris)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    Pv4CL1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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