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Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_PP7-tag
Plasmid#73078Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-endDepositorInsertE2F7 (E2F7 Human)
UseTags5'-PP7-tag RNAExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by intronsPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/E2F2/NRF-1(-64)-like
Plasmid#66742Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGCG(-64) to TACAPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
161+80pCalb2/mutCREB(-2)-like
Plasmid#66751Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGCGC (-2) to TAGAPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/mutNRF-1(-41)-like
Plasmid#66744Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGC (-41) to ATTPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/dmutNRF-1(-41;-35)-like
Plasmid#66741Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGCAGGCGC (-41;-35) to ATTAGGATTPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_2ntmut
Plasmid#73074Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding siteDepositorInsertE2F7 (E2F7 Human)
UseTagsExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns, 2…PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-V18D-mCherry
Plasmid#193046PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationV18D, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-C290S-mCherry
Plasmid#193047PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC290S, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-cbm_PCaspase
Plasmid#214047PurposeBacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
UseTagsExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterlacUV5 promoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase-N-C-term
Plasmid#214046PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
UseTagsExpressionBacterialMutationaa 1-153, 154-666PromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-sm_PCaspase
Plasmid#214048PurposeBacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
UseTagsExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEcNucC
Plasmid#214050PurposeBacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorInsertEcNucC
UseTagsExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT
Plasmid#214030PurposeBacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceumDepositorInsertSAVED-CHAT
UseTagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-dSAVED-CHAT
Plasmid#214031PurposeBacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
UseTagsStrep-tag IIExpressionBacterialMutationH375A, C422APromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-sm
Plasmid#214033PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
UseTagsStrep-tag IIExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCaspase
Plasmid#214034PurposeBacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
UseTagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1213 - pL0_p16OMT (pro + 5U)
Plasmid#203890Purpose16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsert16OMT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro
Plasmid#211821PurposePiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishmentDepositorInsertSpCas9-ABE8e, tracrRNA and Puromycin resistance gene
UseTagsExpressionMammalianMutationPromoterchicken β-actin promoter, U6 promoter and hPGK pr…Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lamp1 FLAG
Plasmid#199750PurposeLentiviral construct to stably express Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only