We narrowed to 27,491 results for: sta

1. pCMV-Frankenbody-FLAG-mRuby2

   Plasmid

   #175294

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mRuby2
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pX601-GFP

   Plasmid

   #84040

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with GFP

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPN1-gamma-tubulin

   Plasmid

   #87956

   Purpose

   Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene …

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBK1

   Plasmid

   #87443

   Purpose

   express TBK1 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   TBK1 (Tbk1 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdTrack: shRev-Erbalpha

   Plasmid

   #22749

   Depositor

   Bruce Spiegelman

   Article

   Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

   Insert

   shRev-Erbalpha (Nr1d1 Mouse)
   

   Use

   Adenoviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Frankenbody-FLAG-mEGFP

   Plasmid

   #175293

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI-N1ICD

   Plasmid

   #185525

   Purpose

   Constitutive overexpression of human NOTCH1 intracellular domain

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-hKif5b(1-560)rigor-2xmNeongreen

   Plasmid

   #174649

   Purpose

   Live-cell marker for stable microtubules

   Depositor

   Lukas Kapitein

   Article

   Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

   Insert

   hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
   

   Tags

   mNeongreen-mNeongreen

   Expression

   Mammalian

   Mutation

   rigor mutation G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-HBA-RIBEYE-eGFP

    Plasmid

    #241988

    Purpose

    Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance

    Depositor

    Tobias Moser

    Article

    Kapoor et al eLife13:RP98254

    Insert

    RIBEYE-GFP (CTBP2 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    hybrid CMV-enhancer-human-beta-actin promoter (CM…

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601-mCherry

    Plasmid

    #84039

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with mCherry

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB2B

    Plasmid

    #195284

    Purpose

    Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Puro
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN-A112C-GpA

    Plasmid

    #191238

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Frankenbody-FLAG-Halo

    Plasmid

    #175296

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Expression

    Bacterial

    Promoter

    PA1

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tn7 integration plasmid

    Plasmid

    #154134

    Purpose

    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Inserts

    left and right end of Tn7 arm
    Spectinomycin resistant gene
    

    Expression

    Bacterial

    Promoter

    Spectinomycin resistance gene promoter and no

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-1 shRev-Erbalpha

    Plasmid

    #22747

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only