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pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

Plasmid

#205276

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

T513K

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

Plasmid

#205274

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D487K

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

Plasmid

#205272

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D300R

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-4SA(S61)

Plasmid

#33080

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S109A, S127A, S164A, S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad3 (146-425)

Plasmid

#14966

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 146-425

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
3xFN-Tel-TAL/pCMV-7.1

Plasmid

#63589

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

Plasmid

#74263

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.R404X truncation

Promoter

CMV

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
p6897 PHAGE-P CMVT N-HA-hE6AP III wt

Plasmid

#37605

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV tet regulatable

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV TIP60 365-513

Plasmid

#78796

Purpose

To overexpress TIP60 365-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

aa365-513

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE-CMV-Flag-RIOK3-STOP_IDG-K

Plasmid

#170576

Purpose

Gateway destination clone of RIOK3 (human) tagged with N-terminal Flag for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

Flag-RIOK3 (RIOK3 Human)

Use

Lentiviral; Gateway destination

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-4SA(S127)

Plasmid

#33082

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S61A, S109A, S164A, S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
CMV-CRY2oligo-mcherry-ANXA11(R346C)

Plasmid

#164206

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R346C

Promoter

CMV

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

Plasmid

#155195

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 276-315

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-LC3B G120

Plasmid

#123116

Purpose

Expresses EGFP-LC3B G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S811

Plasmid

#58919

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S811 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-hAR-W433A,L436A,F437A

Plasmid

#89086

Purpose

mammalian expression of human androgen receptor with mutations of N-terminal WxxLF motif

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR WxxLF mutant (AR Human)

Use

Tags

Expression

Mammalian

Mutation

hAR-W433A,L436A,F437A

Promoter

CMV

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-140)

Plasmid

#64675

Purpose

expresses only N terminus of RNF8, amino acids 1 to 140

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

only N terminus amino acids 1-140

Promoter

CMV

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only