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pCW-12S-E1A-HA-puro
Plasmid#212671PurposeExpress adenovirus E1ADepositorInsert12S-E1A
UseLentiviralTagsHAExpressionMutationPromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-hCDC6p-Venus
Plasmid#212665PurposeExpress Venus under CDC6 promoterDepositorInserthCDC6p-Venus
UseLentiviralTagsExpressionMutationPromoterhCDC6pAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR95(URA3)
Plasmid#203917PurposeBLINCAR plasmid for gene deletion, URA3 targetDepositorInsertsURA3 upstream homology
URA3 downstream homology
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Noodles Plasmid
Plasmid#212613PurposeAn all-in-one system for on-target efficiency analysis of CRISPR guide RNAsDepositorTypeEmpty backboneUseCRISPR and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-AqpZ-ALFA
Plasmid#212310PurposeExpression of AqpZ with ALFA tagDepositorInsertAquaporin Z
UseTagsALFA tag and Strep-Tag IIExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Tgl3-4V5-APEX2-URA
Plasmid#207059PurposeExpressing Tgl3-4V5-APEX2. Insufficient signal for EM.DepositorInsertTgl3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpTGL3Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Pex3-4V5-APEX2-URA
Plasmid#207057PurposeExpressing Pex3-4V5-APEX2. Insufficient signal for EM.DepositorInsertPEX3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpPEX3Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHFdist_rm
Plasmid#162318PurposeP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removedDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationIHF distal site is removedPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB40
Plasmid#205136PurposeIntegrating expression of SnxA-GFP in DictyosteliumDepositorInsertSnxA
UseDictyostelium expressionTagsEGFPExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR95
Plasmid#203916PurposeBLINCAR plasmid for gene deletion, no genomic targetDepositorInsertmodified MCS
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pProbe_CUSPER
Plasmid#185953Purposebroad host plasmid that can be used to construct reproductive success bioreporterDepositorInsertUnspecified
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-Cas9
Plasmid#186723PurposeTransient expression of Cas9 in plant cells.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_hTLN2-head
Plasmid#164836PurposeExpression of human talin2 headDepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-GFP-ePDZ-CAAX
Plasmid#135603PurposeExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ-CAAX
UseIn vitro transcription (mrna synthesis)TagsCAAX, EGFP, and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-EMTB-GFP-ePDZ
Plasmid#135600PurposeExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertEMTB-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-membrane-mCherry-LOV
Plasmid#135599PurposeExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertmembrane-mCherry-LOV
UseIn vitro transcription (mrna synthesis)TagsLOV and mCherryExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP-ePDZ
Plasmid#135602PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-nolinker-GFP
Plasmid#108579PurposeExpresses PKAcs-GFP fusion in mammalian cellsDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-G12X-GFP
Plasmid#108588PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
MPS-1-cmyc
Plasmid#113059PurposeExpresses worm Myc-tagged MPS-1 in mammalian cellsDepositorInsertMiRP K+ channel accessory Subunit 1 (mps-1 Nematode)
UseTagsc-MycExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_C83D
Plasmid#113048PurposeExpression of mutant hSDH5DepositorInserthSDH5_C83D (SDHAF2 Human)
UseTagsExpressionBacterialMutationC83D, Insert begins at amino acid 39PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn1r105
Plasmid#105994PurposeTo prepare riboprobe for in situ hybridizationDepositorUseTo generate riboprobeTagsExpressionMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pstb-LAFR5-exoY
Plasmid#86170Purposepstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/BamDepositorInsertexoY
UseTagsExpressionBacterialMutationPromoterSmb21651Available sinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBN279
Plasmid#86725PurposeTissue-specific FLP expression plasmid for C. elegansDepositorInsertPhlh-8::FLP D5
UseTagsExpressionWormMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP4GY-cccdB
Plasmid#83878PurposeDestination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pbx2
Plasmid#74351PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx2. Not for gene expression.DepositorInsertpre B cell leukemia homeobox 2 (Pbx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Zhx3
Plasmid#74366PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx3. Not for gene expression.DepositorInsertzinc fingers and homeoboxes 3 (Zhx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Zhx2
Plasmid#74365PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx2. Not for gene expression.DepositorInsertzinc fingers and homeoboxes 2 (Zhx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Zfhx3
Plasmid#74363PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx3. Not for gene expression.DepositorInsertzinc finger homeobox 3 (Zfhx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Tgif1
Plasmid#74359PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Tgif1. Not for gene expression.DepositorInsertTGFB-induced factor homeobox 1 (Tgif1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Six1
Plasmid#74358PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.DepositorInsertsine oculis-related homeobox 1 (Six1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pbx3
Plasmid#74352PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.DepositorInsertpre B cell leukemia homeobox 3 (Pbx3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-Histone H2B
Plasmid#45085DepositorInsertHistone H2B
UseRNAi; In vitro transcriptionTagsExpressionMutationPromoterT7 sense, T3 antisenseAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-Histone H2A
Plasmid#45084DepositorInsertHistone H2A
UseRNAi; In vitro transcriptionTagsExpressionMutationPromoterT7 sense, T3 antisenseAvailable sinceMay 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-gH4
Plasmid#45081DepositorInsertgerminal Histone H4
UseRNAi; In vitro transcriptionTagsExpressionMutationPromoterT7 sense, T3 antisenseAvailable sinceMay 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pDD2045
Plasmid#101379PurposeName: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 1
Plasmid#183828PurposeExpress EML4-ALK fusion variant 1 (E13;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-13 and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N/D1203N
Plasmid#183833PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171N/D1203NPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a
Plasmid#183829PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScaffold-H1 donor
Plasmid#118152PurposePCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A Aα full length
Plasmid#132634Purposeexpresses human PP2A Aα full length in E. coliDepositorInsertPP2A Aα (PPP2R1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON
Plasmid#180423PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSASDepositorInsertSpike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A B56α full length
Plasmid#132635Purposeexpresses human PP2A B56α full length in E. coliDepositorInsertPP2A B56α
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON
Plasmid#180424PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intactDepositorInsertSpike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 OPTN TSS-guide4
Plasmid#118199PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.617.2.v1
Plasmid#182575PurposeMammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950NDepositorInsertSpike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only
