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Plasmid#63947PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only
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pGBW-m4252811
Plasmid#153563PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
AKAP13
Plasmid#73232PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAKAP13 (AKAP13 Human)
UseTagsHis-6-TEVExpressionBacterialMutationcontains amino acids 1976-2211PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133578
Plasmid#152329PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4252962
Plasmid#153660PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4252852
Plasmid#153644PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4252912
Plasmid#153704PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc4-CBPHISNDHFR
Plasmid#63999PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Calmodulin Binding Peptide (CBP); TEV…ExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EPB41
Plasmid#32040PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPB41 (EPB41 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationFERM domain only (amino acids 1-279)PromoterAvailable sinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134122
Plasmid#152107PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;CBPExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133241
Plasmid#152126PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TRXExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSKB3.TM1777
Plasmid#11421DepositorInsertNusA (nusA T. maritima)
UseTags6x His and TEV protease siteExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-HPCHISNDHFR
Plasmid#64002PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRPF1 (3MO8)
Plasmid#28107PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRPF1:JMC014-E01:C44383 (BRPF1 Human)
UseTagsHis-tag with integrated TEV protease siteExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENND1A (DENND1AA-c032)
Plasmid#110270PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDENND1A (DENND1A Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP972
Plasmid#98806PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
UseTagsExpressionBacterialMutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc2-HPCHISNDHFR
Plasmid#63966PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Protein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBACgus N-His/GST/LRRK2 1867-2176
Plasmid#40406DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867-2176PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits