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Showing: 3921 - 3940 of 20444 results

  3. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  5. pJB06

    Plasmid
    #190480
    Purpose
    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Cas9
    XylR
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    xylR-driven promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190480-plasmid-map-sequence-id-374623

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  6. qTAG-C-miniTurbo-Zeo

    Plasmid
    #207736
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207736-plasmid-map-sequence-id-413784

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  7. qTAG-N-Zeo-miniTurbo

    Plasmid
    #207703
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207703-plasmid-map-sequence-id-413764

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  9. pDB4282

    Plasmid
    #98701
    Purpose
    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98701-plasmid-map-sequence-id-182827

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  10. pDB4283

    Plasmid
    #98702
    Purpose
    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98702-plasmid-map-sequence-id-182828

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  11. qTAG-C-mNeon-Blast

    Plasmid
    #207709
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207709-plasmid-map-sequence-id-413806

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  13. qTAG-C-3xHA-Blast

    Plasmid
    #207718
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207718-plasmid-map-sequence-id-413775

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  14. qTAG-N-Zeo-dTAG

    Plasmid
    #207705
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207705-plasmid-map-sequence-id-413766

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  15. qTAG-C-V5-Zeo

    Plasmid
    #207741
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207741-plasmid-map-sequence-id-413810

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  16. qTAG-N-Zeo-3xFLAG

    Plasmid
    #207706
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207706-plasmid-map-sequence-id-413767

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  17. qTAG-N-Blast-3xHA

    Plasmid
    #207685
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207685-plasmid-map-sequence-id-413800

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  20. qTAG-C-moxGFP-Puro

    Plasmid
    #207721
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207721-plasmid-map-sequence-id-413798

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Showing: 3921 - 3940 of 20444 results