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pcDNA3-Flag-Trib2

Plasmid

#131155

Purpose

Mammalian expression of flag-tagged mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3037(XI-5 MarkerFree)

Plasmid

#73277

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTW088

Plasmid

#115936

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 3'UTR No E1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW094

Plasmid

#115937

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 E1-3'UTR End

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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PatoG-OG241

Plasmid

#72839

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoG

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoG

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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hFlt1 Y1333F

Plasmid

#86037

Purpose

human VEGFR1 with Y to F mutation at position 1333

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 Y1333F (FLT1 Human)

Use

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

Y1333F; HA tag is inserted downstream of signal …

Promoter

CMV

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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hFLT1 delta 754-763

Plasmid

#86035

Purpose

human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 delta754-763 aa (FLT1 Human)

Use

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

delta 754-763

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEVOL_PylRS(WT)-tRNA

Plasmid

#223512

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Inserts

Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
tRNA Pyl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD, rrnB, and glnS and proK

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Use

Tags

3xFLAG and GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE

Plasmid

#225349

Purpose

Lentiviral vector for Wnt3a and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Human, Synthetic)

Use

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

Plasmid

#209922

Purpose

A knock-out vector for the dog CDH1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CDH1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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(attB) BRaf with MS2-circRNA

Plasmid

#226164

Purpose

Used to integrate HA-tagged BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

2xHA-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-mCherry-mDYNLL1

Plasmid

#210336

Purpose

Overexpression of mDYNLL1

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mDYNLL1 (Dynll1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Use

Tags

Expression

Bacterial

Mutation

A28C in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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