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Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#219782PurposeCaST-IRES for HEK cell expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-MK1-EHMT2-V5
Plasmid#31113DepositorInserteuchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationStop codon mutated to AlaPromoterAvailable sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJL-SaKKH-BE3
Plasmid#85170PurposeExpresses SaKKH-BE3 in mammalian cells [Edit]DepositorInsertSaKKH-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCWX-UBI-hTert-PGK-BSD
Plasmid#114316PurposeConstitutive expression of the hTert proteinDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2568
Plasmid#142120PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTGIF1 (TGIF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3126
Plasmid#144602PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a
Plasmid#80611PurposepBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pP2A-mCherry-N1
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-mCherry
Plasmid#185474PurposeMammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_429
Plasmid#179098PurposeBE Cas expressionDepositorInsertABE8e (Cas9-NG)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HLCS_pCSdest
Plasmid#53803DepositorInsertHLCS (HLCS Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE
Plasmid#101852PurposeDirect reprogramming of adult fibroblasts to neuronsDepositorInsertAscl1, Brn2
UseLentiviralTagsExpressionMutationPromoterU6, PGKAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B11-WT-FLAGC
Plasmid#161903PurposeExpresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInserthydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
UseTagsFLAG-GFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
UseTagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-survivin
Plasmid#196908Purposevisualization of midzone on anaphase and midbody later during mitosis in living mammalian cellsDepositorInsertsurvivin
UseTagsEGFPExpressionMammalianMutationE129KPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
hACE2
Plasmid#1786PurposeMammalian expression of human ACE2DepositorUseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_ADAR2DD_E488Q
Plasmid#205598Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cellsDepositorInsertADAR2 deaminase domain (ADARB1 Human)
UseTagsExpressionMammalianMutationE488Q hyperactive mutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FlpO
Plasmid#89574PurposeExpresses FlpO under pCAGDepositorInsertFlpO
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-ATF5-PROX1-HNF1A
Plasmid#149725Purposeexpressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)DepositorInsertATF5-PROX1-HNF1A (ATF5 Human)
UseLentiviralTagsP2A, T2AExpressionMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC
Plasmid#125695PurposeGlobal labeling of inhibitory synapses (red fluorescence)DepositorInsertmScarlet-Gephyrin.FingR-IL2RGTC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-GFP
Plasmid#183906PurposeExpression of GFP under the human GFAP ABC1D promoterDepositorInsertGFP (GFAP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Luc2-IRES-ZsGreen-W
Plasmid#164432PurposeLentiviral vector backbone encoding firefly luciferase and ZsGreenDepositorInsertsLuciferase
ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetON-3G
Plasmid#96963PurposeMammalian expressiom of Clontech rtTA driven by human beta-actine promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-i53
Plasmid#92170Purposerecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1DepositorInserti53
UseAAVTagsFLAGExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJuly 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
murine TCR OTII-2A.pMIG II
Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
Plasmid#172506PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloningDepositorInsertDisCas7-11 bacterial expression vector with golden gate site for guides
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF1894
Plasmid#144200PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAHRR (AHRR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS HSPA1A
Plasmid#19537DepositorInsertheat shock 70kDa protein 1A (HSPA1A Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF2195
Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIPL (MLXIPL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Egr1
Plasmid#11729DepositorInsertEgr-1 (Egr1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA
Plasmid#166693PurposeExpress dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.DepositorInsertsdCas9-VPR
3x Cnga1 promoter-targeting sgRNAs
UseCRISPRTagsExpressionMammalianMutationPromoterTRE and U6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only