We narrowed to 39,261 results for: lat

1. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSV-CaMKIV(1-313)

   Plasmid

   #45063

   Purpose

   Expression of constit active CaMKIV

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   Cam Kinase IV(1-313) (Camk4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncated at amino acid 313*

   Promoter

   RSV

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DCX(S306D)-2A-mCherry

   Plasmid

   #118292

   Purpose

   Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   Doublecortin (DCX Human)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Mutation

   Serine 306 changed to Aspartic Acid

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DCX(S306A)-2A-mCherry

   Plasmid

   #118291

   Purpose

   Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanine

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   Doublecortin (DCX Human)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Mutation

   changed Serine 306 to Alanine

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD226-bio-His

   Plasmid

   #51646

   Purpose

   Expresses full-length CD226 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD226 (CD226 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYFP-ErbB2ΔC990

   Plasmid

   #66946

   Purpose

   Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

   Depositor

   Bo van_Deurs, Mads Lerdrup

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Use

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   Deletion of C990. (see depositor comment below on…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-EGFP-AC8(1-106)

   Plasmid

   #181958

   Purpose

   Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   EGFP-AC8(1-106) (Adcy8 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYFP-ErbB2ΔC776

   Plasmid

   #66947

   Purpose

   Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

   Depositor

   Bo van_Deurs, Mads Lerdrup

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Use

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   Deletion of C776 (see depositor comment below on …

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCK668

   Plasmid

   #192637

   Purpose

   Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dCas9-NG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dCas9-NG ha…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLK2

    Plasmid

    #155459

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLK2 (CLK2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ECE1-His-bio

    Plasmid

    #53361

    Purpose

    Expresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ECE1 (ECE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBpuro - HA KLF4 FL

    Plasmid

    #34589

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIE-hα2M-H6

    Plasmid

    #128492

    Purpose

    Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    IE1

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1hLCAD_FLAG

    Plasmid

    #24491

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (ACADL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL5.0 mCherry-hCortKD-Flcortmut3YD

    Plasmid

    #187266

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL5.0 hCortKD-Flcortmut3YF mCherry

    Plasmid

    #187265

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    3YF (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH728-CEN-RLuc/maxCFLuc

    Plasmid

    #38212

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRC/CMV-TYK2K930R-VSV

    Plasmid

    #139346

    Purpose

    Expression of kinase-dead TYK2-K930R protein

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-K930R

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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