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Showing: 3901 - 3920 of 5091 results

  8. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  9. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

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  10. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

    Plasmid
    #108373
    Purpose
    Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108373-plasmid-map-sequence-id-208350

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  14. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107576
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107576-plasmid-map-sequence-id-214314

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  15. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid
    #198569
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198569-plasmid-map-sequence-id-438870

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  16. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid
    #182425
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    Use
    Tags
    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182425-plasmid-map-sequence-id-360468

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Showing: 3901 - 3920 of 5091 results