Addgene

We narrowed to 5,232 results for: arl

Showing: 3901 - 3920 of 5232 results

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pAAV-double floxed-eNpHR-EYFP-WPRE-pA

Plasmid

#20949

Purpose

Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

halorhodopsin

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eNpHR 3.0-EYFP

Plasmid

#26970

Purpose

3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Insert

eNpHR 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS), ER Export Signal (FCYENE)

Promoter

CaMKIIa

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

Plasmid

#126081

Purpose

vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation

Depositor

Karl Deisseroth

Insert

hChR2(H134R)-eYFP

Use

AAV

Tags

eYFP

Expression

Mutation

H134R

Promoter

Ef1a

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0

Plasmid

#126080

Purpose

sCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientation

Depositor

Karl Deisseroth

Insert

hChR2(H134R)-eYFP

Use

AAV

Tags

eYFP

Expression

Mutation

H134R

Promoter

Ef1a

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Pcamr-RFP

Plasmid

#190614

Purpose

Expresses RFP with an engineered CamR-regulated promoter in E.coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al ACS Synth Biol. 2022 Jan 5. doi: 10.1021

Insert

mScarlet-i

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

Plasmid

#100050

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

hChR2(E123T/H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

humanized, E123T/H134R

Promoter

CaMKIIa

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

Plasmid

#100061

Purpose

AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

EF1a

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

Plasmid

#100049

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

hChR2(E123T/H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

humanized, E123T/H134R

Promoter

hSynap

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pNCMV

Plasmid

#37453

Depositor

Karl Munger

Article

Spangle et al J Virol. 2010 Sep;84(18):9398-407. Epub

Type

Empty backbone

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
GBH-2aG4-insBFP.1

Plasmid

#188025

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker insulin BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-2aG4-insGFP.1

Plasmid

#188028

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-2aG4-insGFP.2

Plasmid

#188029

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-R-insBFP.2

Plasmid

#188008

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted mRFP protein trap; secondary marker insulin BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-R-insBFP.3

Plasmid

#188009

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted mRFP protein trap; secondary marker insulin BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-R-insGFP.1

Plasmid

#188010

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted mRFP protein trap; secondary marker insulin GFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-R-insGFP.2

Plasmid

#188011

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted mRFP protein trap; secondary marker insulin GFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-R-insGFP.3

Plasmid

#188012

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted mRFP protein trap; secondary marker insulin GFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; insulin promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-2aG4-cmlc2BFP.1

Plasmid

#188019

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; cmlc2 promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-2aG4-cmlc2BFP.2

Plasmid

#188020

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; cmlc2 promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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GBH-2aG4-cmlc2BFP.3

Plasmid

#188021

Purpose

Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

Depositor

Karl Clark

Insert

Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP

Use

Targetable insertional mutagen and reporter system

Tags

Expression

Mutation

Promoter

Endogenuous Promoter; cmlc2 promotor

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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