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Plasmid#154793PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133151
Plasmid#153581PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133806
Plasmid#153713PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAT11
Plasmid#112592PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneUseTags6His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HISNDHFR
Plasmid#63958PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4252875
Plasmid#153789PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5UBB (LOC149281)
Plasmid#101257PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL11B (METTL11B Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LMNB2
Plasmid#36896PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLMNB2 (LMNB2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains Laminb2 Ig-like domain, amino acid resid…PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsStrep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
5VC8 (WHSC1)
Plasmid#101262PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWHSC1 (NSD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133341
Plasmid#152285PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceSept. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ANKRA2
Plasmid#36904PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertANKRA2 (ANKRA2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acid residues 148-313 (ANK repeats)PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc3-FLAGNDHFR
Plasmid#63979PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EPHB3
Plasmid#28165PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHB3 (EPHB3 Human)
UseTagsHis tag with TEV cleavage and Honeybee melittin S…ExpressionBacterialMutationLigand binding domain (residues 39-211).PromoterAvailable sinceMarch 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSKB3.MPN348
Plasmid#11437DepositorInsertmethenyltetrahydrofolate synthetase
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133930
Plasmid#152574PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 18, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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RECQL5 (RECQL5A-c104)
Plasmid#98234PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4252828
Plasmid#153693PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits