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Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
DN1-Gli3 in AINGpCAGGS
Plasmid#121970Purposemedium Gli3 transcriptional activatorDepositorInsertDN2Gli3 (GLI3 Human)
UseChick electroporationTags5xmycExpressionMammalianMutationN terminus deletion (nt1-864)PromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK2 K235R
Plasmid#80872Purposemammalian expression of ALK2 K235RDepositorInsertALK2 (ACVR1 Human)
UseTagsHAExpressionMammalianMutationK235RPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
3019_pETcon-SARS-CoV-2-RBD_E484K
Plasmid#184404Purposeyeast surface display of the SARS-CoV-2 Eta variant RBDDepositorInsertSARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationE484KPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ZKSCAN1_Tau7-12WT
Plasmid#194166PurposeInsert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.DepositorInsertMicrotubule-associated protein tau 7-12 WT
UseTags3X FlagExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ZKSCAN1_Tau10-12WT
Plasmid#194163PurposeInsert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.DepositorInsertMicrotubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
UseTags3X flag tagExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
UseTagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…PromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorInsertRFPnols-PQR-ZeocinR (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-dKARD
Plasmid#114053Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-dKARD (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance): AGATACTAGCT…PromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y
Plasmid#184406Purposeyeast surface display of the SARS-CoV-2 Beta variant RBDDepositorInsertSARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationK417N+E484K+N501YPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-838;+80pCalb2
Plasmid#66750Purposeluciferase reporter for Calb2 promoter (-838 to +80, WT)DepositorInsertCalb2 promoter (-838bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-2xFLAG
Plasmid#130979PurposeExpression of KIF1C-2xFLAG in mammalian cells.DepositorInsertKIF1C-2xFLAG (KIF1C Human)
UseTags2xFLAGExpressionMammalianMutation4 silent mutations that make this construct resis…PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DHFR.cHSF1
Plasmid#134738PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable sinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Calb2-3'UTR
Plasmid#68363PurposeLuciferase miRNA Target Expression VectorDepositorInsertCalb2 3'UTR (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationC to T change in UTRPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-CALB2-3'UTR mir-30 mut2
Plasmid#74429PurposeCalb2 UTR with mutated mir-30 site 1 and 2DepositorInsertCALB2 3'UTR (CALB2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R2pH-LAMP-FRB
Plasmid#225138PurposeR2pH-LAMP1-labelled lysosomes with FRBDepositorInsertLamp1 (Lamp1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
R2pH-LAMPx3ALFA
Plasmid#225136PurposeR2pH-LAMP1-labelled lysosomes with 3xLFADepositorInsertLamp1 (Lamp1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
eft-3p:act-5(ptc):tbb2 3' UTR
Plasmid#202331PurposeThe ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.DepositorInsertseft-3 promoter
act-5(ptc)
tbb-2 3' UTR
UseTagsbase 238 C -> A mutation causing a PTC at codo…ExpressionWormMutationPromoterThis is a promoter sequence for C. elegans eft-3,…Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK7188
Plasmid#219768PurposeVector for expression Pv4CL1 in yeast (P.pastoris)DepositorInsertPv4CL1
UseTagsExpressionYeastMutationPromoterpGAPAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only