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pmCherry-EGFP-N3-CYB5R3 K214R

Plasmid

#204570

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

K214R

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-3xFLAG-CMV14-CYB5R3

Plasmid

#204551

Purpose

Expresses 3xFLAG-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3

Plasmid

#204569

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-GABARAP

Plasmid

#204574

Purpose

Expresses Ash-tagged GABARAP in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

GABARAP (GABARAP Human)

Use

Tags

Expression

Mammalian

Mutation

deltaL117

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB Ubiqutin-BFP

Plasmid

#202426

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

Ubiquitin, TagBFP

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1

Plasmid

#197395

Purpose

Expresses SUMO-tagged mouse METAP1

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 (Metap1 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD

Plasmid

#148514

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD

Plasmid

#148515

Purpose

Bacterial Expression of DmThor_50-63-DmeIF4Ga_631-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T

Plasmid

#147590

Purpose

Insect Expression of DmThor-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CG32016-E (Thor Fly)

Use

Tags

Expression

Insect

Mutation

DmThor two silent mutations compared to the seque…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T

Plasmid

#147591

Purpose

Insect Expression of DmThor-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmCup_417-1133 (Thor Fly)

Use

Tags

Expression

Insect

Mutation

Thor contains two silent mutations A246C and T321…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T

Plasmid

#147592

Purpose

Insect Expression of DmThor-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmeIF4Ga_687-1666 (Thor Fly)

Use

Tags

Expression

Insect

Mutation

DmThor two silent mutations A246C and T321C compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free NES

Plasmid

#182482

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
NES

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10 and GAL7

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free ClLIS1 (M)

Plasmid

#182484

Purpose

Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS(M)
LS

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10 and GAL7

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DDX27

Plasmid

#155513

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX27 (DDX27 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-EGFP-eDHFR(69K6)-cRaf

Plasmid

#178849

Purpose

Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-cRaf (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-cRaf-mNG-eDHFR(69K6)

Plasmid

#172107

Purpose

Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR 240-546

Plasmid

#158108

Purpose

reporter 3'UTR hRBM8A (short)

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

3'UTR of hRBM8A (RBM8A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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