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Plasmid#141253PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only
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pSpCas9(BB)-2A-Blast_HP1beta_gRNA
Plasmid#127908PurposeWT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta geneDepositorInsertgRNA for Human 5' HP1beta
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335 Mouse 5' Srcap gRNA A
Plasmid#127904PurposeCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap geneDepositorInsertSrcap gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 3' Ubf gRNA
Plasmid#127902PurposeWT Cas9 Vector targeting the 3' end of the mouse Ubf geneDepositorInsertgRNA for Mouse 3' Ubf
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-U6-myogenin stop codon gRNA
Plasmid#69549PurposeExpresses guide RNA targeting near the stop codon of myogenin in the mouse genome.DepositorInsertMyogenin C terminus-targeting guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZSCAN18-donor
Plasmid#113821PurposeCRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFPDepositorInsertZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF561-donor
Plasmid#113801PurposeCRISPR donor plasmid to tag human transcription factor ZNF561 with GFPDepositorInsertZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF700-donor
Plasmid#112385PurposeCRISPR donor plasmid to tag human transcription factor ZNF700 with GFPDepositorInsertZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF169-donor
Plasmid#112376PurposeCRISPR donor plasmid to tag human transcription factor ZNF169 with GFPDepositorInsertZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFP1-donor
Plasmid#112374PurposeCRISPR donor plasmid to tag human transcription factor ZFP1 with GFPDepositorInsertZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF227-donor
Plasmid#112368PurposeCRISPR donor plasmid to tag human transcription factor ZNF227 with GFPDepositorInsertZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTX1-donor
Plasmid#112364PurposeCRISPR donor plasmid to tag human transcription factor OTX1 with GFPDepositorInsertOTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF175-donor
Plasmid#112347PurposeCRISPR donor plasmid to tag human transcription factor ZNF175 with GFPDepositorInsertZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF354C-donor
Plasmid#112339PurposeCRISPR donor plasmid to tag human transcription factor ZNF354C with GFPDepositorInsertZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF347-donor
Plasmid#112326PurposeCRISPR donor plasmid to tag human transcription factor ZNF347 with GFPDepositorInsertZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-dTAG
Plasmid#207694PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBA560
Plasmid#186236PurposepTet-eLbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRTagsExpressionYeastMutationPromoter5S rRNA-tRNA(Arg)Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only