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Plasmid#189609PurposeRetroviral expression vector for IL11DepositorInsertIL11 (IL11 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA
Plasmid#20296PurposeCre-activated AAV expression of EYFPDepositorInsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL8
Plasmid#189608PurposeRetroviral expression vector for IL8DepositorInsertIL8 (CXCL8 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS
Plasmid#105649PurposetdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS_MCS
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-FLAG-HA-T(WT)
Plasmid#181737Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceAug. 3, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Phage-PGK-GFP-T(WT)
Plasmid#181736Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pT7CFE1_EVA71_VP12A
Plasmid#212977PurposeEncodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extractsDepositorInsertEnterovirus A71 4643 VP12A
UseMammalian cell free protein expressionTagsHA tagExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVD68_VP12A
Plasmid#212984PurposeEncodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extractsDepositorInsertEV-D68 MO/14/18947 VP12A
UseMammalian cell free protein expressionTagsHAExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVD68_3ABC
Plasmid#212979Purposencodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extractsDepositorInsertEV-D68 MO/14/18947 3ABC
UseMammalian cell free protein expressionTagsHAExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_EVD68_3ABCD
Plasmid#212981PurposeEncodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extractsDepositorInsertEV-D68 MO/14/18947 3ABCD
UseMammalian cell free protein expressionTagsHAExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAS9id_TRP
Plasmid#141254PurposeGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAS9i_TRP
Plasmid#141253PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-U6-myogenin stop codon gRNA
Plasmid#69549PurposeExpresses guide RNA targeting near the stop codon of myogenin in the mouse genome.DepositorInsertMyogenin C terminus-targeting guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eNpHR 3.0-EYFP
Plasmid#26972PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibitionDepositorHas ServiceAAV5InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eNpHR 3.0-EYFP
Plasmid#26971PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorHas ServiceAAV1 and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry
Plasmid#26975PurposeAAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryGFP.3
Plasmid#188000PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40
Plasmid#100054PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationH134R mutation in ChR2PromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only