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pCMV-FLAG TIF1gamma M (456-887)

Plasmid

#15735

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma M (TRIM33 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Middle region (amino acids 456-887).

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-10xPP7 oligo-library based

Plasmid

#158199

Purpose

Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of PP7 from the oligo library

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5-Flag-EPLIN delta N-term

Plasmid

#40932

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1-220

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-RA-HRas-IRES2-Lyn-iRFP682

Plasmid

#180584

Purpose

RA-Hras and Lyn-iRFP682

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

RA-Hras and Lyn-iRFP682

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-LOV

Plasmid

#22033

Purpose

Mammalian expression of mVenus tagged LOV2 domain

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-LOV

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Insert length based on Venus (720bp) + LOV (450bp)

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
p2xFlag CMV2 Lats1-PY1 mutant

Plasmid

#18973

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Large tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

PY1 mutant, Tyrosine 376 changed to Alanine

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S163/164A

Plasmid

#33089

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S163A, S164A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-NES-MBP-cATOM(Ras)

Plasmid

#209711

Purpose

Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NES-MBP-cATOM(Ras)

Use

Tags

NES-MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Gab1(PRD)-mCherry-HOTag3

Plasmid

#190026

Purpose

Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

Gab1(PRD) CluMPS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727A-STAT3-3xFlag

Plasmid

#99262

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

S727A

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S78D/S82D

Plasmid

#85187

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S78D/S82D, phosphomimicking mutation

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727D-STAT3-3xFlag

Plasmid

#99263

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

S727D

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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