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Plasmid#174683PurposeExpresses 6xHis-MBP tagged HkCas12aDepositorArticleInserthuHkCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1002
Plasmid#71820PurposeExpress Streptococcus pyogenes Cas9DepositorInsertT7pr_10xHis-MBP-TEV-SPyCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHM6g.AQ_1489
Plasmid#11539DepositorInserttRNA methyltransferase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4252845
Plasmid#153787PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc4-STRHISNDHFR
Plasmid#64000PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046865
Plasmid#153930PurposeBacterial expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
6CCR (GID4)
Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133326
Plasmid#152113PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc4-STRNDHFR
Plasmid#63996PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsStrep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HISNDHFR
Plasmid#63976PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c011)
Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLIC.B4.Ta1145
Plasmid#11533DepositorInsertnicotinate phosphoribosyltransferase (Ta1145 T. acidophilum)
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
DNMT3B (3QKJ)
Plasmid#32044PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDNMT3B (DNMT3B Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPWWP domain only (amino acids 293-442)PromoterAvailable sinceFeb. 22, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pST50Trc4-HISNDHFR
Plasmid#63994PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEMG1 (EMG1 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134207
Plasmid#152431PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4252903
Plasmid#153778PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133141
Plasmid#152404PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits