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Plasmid#184409Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-DLC1-i4
Plasmid#99534PurposeDLC1-isoform 4 expression plasmidDepositorInsertDLC1-isoform 4 (DLC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-N289A-H290A
Plasmid#82032PurposePlasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationEndonuclease-dead mutant: N289A+H290A CtIP; Mutat…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)
Plasmid#75400PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.DepositorInserthCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-S664A-S679A-S745A
Plasmid#82031PurposePlasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationNon phosphorylatable mutations on 3 of the main A…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV KSR2 IRES GFP
Plasmid#25969DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
3159_pETcon-SARS-CoV-2-RBD-L452R-T478K
Plasmid#184407Purposeyeast surface display of the SARS-CoV-2 Delta variant RBDDepositorInsertSARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationL452R+T478KPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h_2ntmut
Plasmid#73067Purposeexpression clone for human mutated SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (E2F7 Human)
UseTagsno tagExpressionMammalianMutation2 nt mutation in AS box that binds E2F7 pre-mRNAPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Mouse, Human)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_PP7-tag
Plasmid#73078Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-endDepositorInsertE2F7 (E2F7 Human)
UseTags5'-PP7-tag RNAExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by intronsPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/E2F2/NRF-1(-64)-like
Plasmid#66742Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGCG(-64) to TACAPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
UseTagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
161+80pCalb2/mutCREB(-2)-like
Plasmid#66751Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGCGC (-2) to TAGAPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/mutNRF-1(-41)-like
Plasmid#66744Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGC (-41) to ATTPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
-161+80pCalb2/dmutNRF-1(-41;-35)-like
Plasmid#66741Purposeluciferase reporter for Calb2 promoter (-161to +80, mutant)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCGCAGGCGC (-41;-35) to ATTAGGATTPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_2ntmut
Plasmid#73074Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding siteDepositorInsertE2F7 (E2F7 Human)
UseTagsExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns, 2…PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-V18D-mCherry
Plasmid#193046PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationV18D, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-C290S-mCherry
Plasmid#193047PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC290S, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-cbm_PCaspase
Plasmid#214047PurposeBacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
UseTagsExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterlacUV5 promoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase-N-C-term
Plasmid#214046PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
UseTagsExpressionBacterialMutationaa 1-153, 154-666PromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only