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pcDNA-PU.1-ETS
Plasmid#124652PurposeChimeric ETS domainDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 48-232 (#182)
Plasmid#99920Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 48-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9-AIDx
Plasmid#135343PurposeExpression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorInsertnSpyCas9-hAIDx
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL113
Plasmid#22471PurposeExpresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.DepositorInsertPhyB NT (PHYB Mouse, Mustard Weed)
UseSynthetic BiologyTags10aaLinker-mCFP-HrasCTExpressionMammalianMutationY276HPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 77-232 (#426)
Plasmid#99921Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 77-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-ST2-com vector
Plasmid#136270PurposeExpresses ABE and ST2-com modified sgRNA scaffoldDepositorInsertABE and sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-SEC61B
Plasmid#207671PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61BDepositorInsertSEC61B (SEC61B Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F3
Plasmid#55786PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)DepositorInsert3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAL130
Plasmid#22475PurposeExpresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF3NT (PIF3 Mouse, Mustard Weed)
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
REEP5-2Strep
Plasmid#210350PurposeExpresses REEP5 fused with 2Strep in mammalian cellsDepositorInsertREEP5 (REEP5 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-MYB-FL
Plasmid#105609Purposetransient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cellsDepositorInsertFull length MYB (mouse) (Myb Mouse)
UseTags3*FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ING2
Plasmid#13294DepositorInsertING2 (INGX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F6
Plasmid#55789PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)DepositorInsert3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-GFP-RGG_withTEVsite
Plasmid#124939PurposeExpresion of RGG_GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationTEV site between RGG and EGFPPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABEMax nSaCas9
Plasmid#135364PurposeExpresion of SaCas9 nickase (D10A) with ABEMax at the N terminusDepositorInsertABEMax nSaCas9
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
Plasmid#137827PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 hML23Cm6
Plasmid#109368PurposeHuman melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6DepositorUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only
