-
Plasmid#96967PurposeExpression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tagDepositorInsertE. coli ArdB
UseTagsN-ter TEV cleavable 6HisExpressionBacterialMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
UseTagsN-ter TEV cleavable 6HIsExpressionMutationN-terminal deletion (AA 1-34)PromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4631
Plasmid#97017PurposeExpressing Pseudomonas aeruginosa orf PA4631 proteinDepositorInsertNucleoside-diphosphate-sugar epimerase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2373
Plasmid#97058PurposeExpressing Sulfolobus solfataricus orf SSO2373 proteinDepositorInsertS. solfataricus orf SSO2373
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO1986
Plasmid#97057PurposeExpressing Sulfolobus solfataricus orf SSO1986 proteinDepositorInsertS. solfataricus orf SSO1986
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPApqsL
Plasmid#97020PurposeExpressing Pseudomonas aeruginosa pqsL proteinDepositorInsertPutative FAD-dependent monooxygenase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSAPPFK
Plasmid#97003PurposeExpressing MRSA PPFK proteinDepositorInsertPutative phosphofructokinase
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSO1404
Plasmid#97056PurposeExpressing Sulfolobus solfataricus orf SSO1404 proteinDepositorInsertS. solfataricus orf SSO1404
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF239
Plasmid#97025PurposeExpressing Pyrobaculum spherical virus orf239 proteinDepositorInsertPSV orf 239
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF165A
Plasmid#97024PurposeExpressing Pyrobaculum spherical virus orf165a proteinDepositorInsertPSV orf 165a
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF137
Plasmid#97023PurposeExpressing Pyrobaculum spherical virus orf137 proteinDepositorInsertPSV orf 137
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA1376
Plasmid#97002PurposeExpressing MRSA sar1376 proteinDepositorInsertPutative 4-oxalocrotonate tautomerase
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF131
Plasmid#97022PurposeExpressing Pyrobaculum spherical virus orf131 proteinDepositorInsertPSV orf 131
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4511
Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSATAG
Plasmid#97004PurposeExpressing MRSA TAG proteinDepositorInsertDNA-3-methyladenine glycosylase I
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationPromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4F2
Plasmid#73431PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.DepositorInsertRepressor 4F2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1B6
Plasmid#73430PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.DepositorInsertRepressor 1B6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3H5
Plasmid#73429PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.DepositorInsertRepressor 3H5 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1D4
Plasmid#73433PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.DepositorInsertRepressor 1D4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1E4
Plasmid#73436PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.DepositorInsertRepressor 1E4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only