Addgene

Showing: 3861 - 3880 of 30473 results

Results per page:

Sort results by:

pAAV-CMV-CBA-hGBA1-HA-hGHpA

Plasmid

#218794

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-HA-hGHpA

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

Plasmid

#74261

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

CMV

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
p2T-CMV-miniSdd6-BE4max-BlastR

Plasmid

#204850

Purpose

For cytosine base editing using miniSdd6 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Polymerase expression - pCMV-Phi29 (LM2708)

Plasmid

#208965

Purpose

Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Phi29(-exo;D169A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-CMV 3xFLAG-GABARAPL2-GFP

Plasmid

#123113

Purpose

Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP217-Lenti-CMV-GFP-WPRE-pA

Plasmid

#62925

Purpose

pLenti vector backbone designed to express GFP from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.3αCaMKII and CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Flag-YAP-S94/F95A

Plasmid

#33057

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S94A, F95A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
p2xFlag CMV2 Lats1-PY1+2 mutant

Plasmid

#18975

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Large tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLU-CMV-Flag-hATG5 E122D

Plasmid

#111804

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E122D

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Promoter

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

Plasmid

#174140

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

Promoter

CMV and T7

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pAdx-CMV-iCre-P2A-tdTomato

Plasmid

#73351

Purpose

Express iCre and tdTomato under CMV promoter

Depositor

Kazuhiro Oka

Insert

CMV-iCre-tdtomato

Use

Adenoviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/myc/Nuc-mutant-hogg1

Plasmid

#18710

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 . 6():235.

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Amino Acid 229 Arginine changed to Glutamine

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG TIF1gamma M (456-887)

Plasmid

#15735

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma M (TRIM33 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Middle region (amino acids 456-887).

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge