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Plasmid#141799PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1086
Plasmid#141791PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2E1 (GTF2E1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1056
Plasmid#141789PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFATC1 (NFATC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0961
Plasmid#141767PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERG (ERG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0941
Plasmid#141762PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertLMO1 (LMO1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0937
Plasmid#141760PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSS18 (SS18 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0936
Plasmid#141759PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSS18 (SS18 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0895
Plasmid#141750PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertING4 (ING4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0811
Plasmid#141715PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDCA7L (CDCA7L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0795
Plasmid#141703PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF7 (ATF7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0414
Plasmid#141611PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0412
Plasmid#141610PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0408
Plasmid#141608PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0411
Plasmid#141609PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0407
Plasmid#141607PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0404
Plasmid#141606PurposeLentiviral vector for overexpressing the CTCFL transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0364
Plasmid#141590PurposeLentiviral vector for overexpressing the TCEB1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCEB1 (ELOC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0362
Plasmid#141589PurposeLentiviral vector for overexpressing the TCEB2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCEB2 (ELOB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d4
Plasmid#182743PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 4 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d13
Plasmid#182745PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 13 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIRC
Plasmid#186343PurposeContains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmClover3-hsLem2
Plasmid#179524PurposeExpress OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoterDepositorInsertLEMD2 (LEMD2 Human)
UseTagsmClover3-mAIDExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-STU
Plasmid#138112PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminatorDepositorInsertFnCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M3_3-pTRNA-scf 2.1 (GB2075)
Plasmid#160560PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInsertM3_3-pTRNA-scf 2.1
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M1_3-pTRNA-scf 2.1 (GB2073)
Plasmid#160558PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertM1_3-pTRNA-scf 2.1
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
KLC1N343S-Myc
Plasmid#166963PurposeExpresses Myc-tagged KLC1 N343S protein in pcDNA3.1DepositorInsertKLC1-N343S (Klc1 Mouse)
UseTagsMycExpressionMammalianMutationKLC1-N343SPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M2_3-pTRNA-scf 2.1 (GB2074)
Plasmid#160559PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInsertM2_3-pTRNA-scf 2.1
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA sCRNA 2.0 (GB2461)
Plasmid#160593PurposeVersion of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.DepositorInsertsgRNA sCRNA 2.0
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)
Plasmid#160625PurposeGB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffoldDepositorInsertU6-26-1gRNA-DFR 2.1
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterU6-26Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)
Plasmid#160570PurposeA version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffoldDepositorInsertsgRNA:scaffold tetraloop MS2 aptamer SAM
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
289aa ELL2
Plasmid#127266PurposeFor in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.DepositorInsertNH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to Ileu. Contains 289…PromoterT7Available sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2 Met133, 138, 186 to Ileu
Plasmid#127269PurposeFor in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 IleuDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu HA tag after M186PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2 Met133, 138, 186 to Ileu
Plasmid#127271PurposeFor in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th MetDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu with HA tag after 8th MetPromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationV107M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M-L273D-L276D
Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationT184M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15D
Plasmid#113010PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutationsDepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to DPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
AL62_pDonor.DMD.TS
Plasmid#100287PurposeDMD intron 43-targeting plasmidDepositorInsertEGFP expression unit (DMD Human, Synthetic)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-N[80]A
Plasmid#96995PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residue 80 was mutated (N[80]A). Mutat…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
UseTagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CD4-YEML-myc
Plasmid#58538PurposeExpresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.DepositorInsertCD4 (CD4 Human)
UseTagsIFITM3 endocytosis Tyrosine-Glutamate-Methionine-…ExpressionMammalianMutationAdded C-terminal Tyrosine-Glutamate-Methionine-Le…PromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)
Plasmid#49075PurposeExpresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC820S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674)
Plasmid#49076PurposeExpresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC1052S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only