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Plasmid#37324DepositorInsertHPV58L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
FDP-P10B2-A75-RZ-URA3
Plasmid#130874PurposeIntegration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tailDepositorInsertmKate
UseExpression of mkate, leu2(q180*), and ura3 on ort…TagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4H5
Plasmid#71716PurposeMembrane scaffold protein (MSP), helix 4+ helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-OSKM
Plasmid#20328PurposeLentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1C
Plasmid#139470PurposeReplacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.DepositorInsertstuffer
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
UseTagsExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC1-P1A1
Plasmid#202842PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1DepositorInsertEGFP/BC1
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-N
Plasmid#108078PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAID-BE3
Plasmid#113417PurposeExpresses hAID-BE3 in mammalian cellsDepositorInserthAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pci-EGFP-UPF1-WT
Plasmid#135994PurposeWT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p206-Switch-ON
Plasmid#217885PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180266PurposeLentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNADepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978DepositorInsertflp
UseSynthetic BiologyTagsExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNLS-iRFP670
Plasmid#45466DepositorInsertiRFP670
UseTagsnuclear localization signalExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NspGFP
Plasmid#40729DepositorInsertN super positive GFP - Leucine Zipper
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173870PurposeMammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 0
Plasmid#206991PurposeDonor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
6BY9 (EHMT1)
Plasmid#119956PurposeBacterial expression for structure determinationDepositorInsertEHMT1 (EHMT1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iDreV
Plasmid#140136Purposecan be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiDreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody (LaM-2)
Plasmid#162276PurposeExpresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.DepositorInsertmCherry-Nanobody (LaM-2)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK522-FAST-PETase
Plasmid#214754Purposeplasmid for expression FAST-PETase in Pseudomonas putidaDepositorInsertFAST-PETase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
UseTagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVEX-CC1
Plasmid#74170Purposeprotein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteriaDepositorInsertDCTN1 (DCTN1 Chicken)
UseTagsNoneExpressionBacterialMutationPromoterInducible Tac promoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-GATA4
Plasmid#46030DepositorInsertGATA4 (GATA4 Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationR43Q, G50S and N197SPromotertetOAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS
Plasmid#12158DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mTurquoise2
Plasmid#99122PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoterDepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorInsertXRCC4 (XRCC4 Human)
UseTagsGFP-2xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His Snk/Plk2
Plasmid#16015DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
p35S:Citrine-TurboID-3xHA
Plasmid#199243PurposeThis plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.DepositorInsertCitrine-TurboID
UseTags3xHA and TurboID-3xHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Bxb1-His
Plasmid#123132PurposeBacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.DepositorInsertBxb1 integrase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDUP3
Plasmid#92174PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG:GPI-GFP
Plasmid#32601DepositorInsertGPI-GFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-UCHL1
Plasmid#22563DepositorInsertUCHL1 (UCHL1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only