-
Plasmid#170102PurposeTransgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.DepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterMouse Hsp68 minimal promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
UseTagsGST- and His10-tagExpressionBacterialMutationWTPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cas9)
Plasmid#174864PurposeExpresses human Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by HsPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCambia1300-PacI
Plasmid#44183DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Grx1-roCherry
Plasmid#141068PurposeFluorescent biosensor for 2GSH/GSSG ratioDepositorInsertGrx1-roCherry
UseTagsExpressionMammalianMutationChanged Alanine 150 to Cysteine, inserted Threon…PromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP
Plasmid#11179PurposeMammalian expression vector for expression of CFPDepositorInsertECFP from Aequorea victoria
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-Cerulean-T2A-FKBP-5-ptase
Plasmid#40897DepositorInsertPM-FRB-Cerulean-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV.CAG.GLPLight1
Plasmid#187467PurposeExpresses GLPLight1 in mammalian cellsDepositorInsertGLPLight1
UseAAVTagsFlag tagExpressionMutationPromoterCAGAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_336
Plasmid#179095PurposeBE Cas expressionDepositorInsertBE3.9 (Cas9-NG)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-PE2
Plasmid#136463PurposeExpresses prime editing 2 (PE2) enzyme upon doxycycline treatmentDepositorInsertPE2
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-BMTU UDG-6xHis
Plasmid#172197Purposeexpresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactionsDepositorInsertBMTU UDG
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pINDUCER21-puro_Gateway-3xFLAG
Plasmid#172981PurposeGateway Destination vector for doxycycline-inducible expression of ORFDepositorTypeEmpty backboneUseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPI
Plasmid#12254Purpose2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsEMCV IRES-EGFP cassetteExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-MYBBP1A
Plasmid#38084DepositorInsertMYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLARRY-EGFP
Plasmid#140025PurposeLentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNADepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only