Addgene

Showing: 3861 - 3880 of 3938 results

Results per page:

Sort results by:

pCEP4CyPet-MAMM

Plasmid

#14033

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Insert

CyPet

Use

Tags

Expression

Mammalian

Mutation

Mammalian optimized CyPet

Promoter

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Stephen Ekker, Daniel Carlson

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Expression

Mutation

Truncate at N and C terminous

Promoter

T3

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 mouse src

Plasmid

#13663

Depositor

Peter Howley, Joan Brugge

Insert

src (Src Mouse)

Use

Tags

Myr

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2133

Plasmid

#72249

Purpose

Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

Y450A, N497A, R661A, Q695A, and Q926A

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-Flag hMcl-1

Plasmid

#25371

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 mouse src K295R Y527F

Plasmid

#13657

Depositor

Peter Howley, Joan Brugge

Insert

src K295R Y527F (Src Mouse)

Use

Tags

Expression

Mammalian

Mutation

K295R Y527F. Dominant negative mutant.

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-FUW-Klf4

Plasmid

#20322

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
miR-146a

Plasmid

#15092

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

pre-miR-146a

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
siSlug3

Plasmid

#10905

Depositor

Bob Weinberg

Article

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

siSlug (SNAI2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-TAP

Plasmid

#12570

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Aug 15. ():.

Type

Empty backbone

Use

Retroviral

Tags

TAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab9 DN

Plasmid

#12676

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
811 pSG5L HA PTEN C124S

Plasmid

#10744

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C124S

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSmOrange-N1

Plasmid

#31898

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
903 pLNCX HA Akt1

Plasmid

#9004

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
shp63alpha pLKO.1 puro

Plasmid

#19120

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p63 (TP63 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
SRa-HA-SUMO1 (Sentrin 1)

Plasmid

#17359

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge