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Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404) (Casp1 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1 (ERAP1 Human)
UseBaculovirus expressionTagsTEV-His10-FlagExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB
Plasmid#92234PurposeExpress wild type prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationnonePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTF101.1gw1
Plasmid#134768PurposeA binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformationDepositorArticleTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133227
Plasmid#152132PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc1-HISNDHFR
Plasmid#63940PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorArticleInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialMutationPromoterT7Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBoCas12a
Plasmid#174673PurposeExpresses 6xHis-MBP tagged BoCas12aDepositorArticleInserthuBoCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0729
Plasmid#162316PurposeTEV protease plasmid for T7-driven E. coli cell-free protein synthesisDepositorInsertT7prom-RBS-NET-TEVprotease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC28-LIC-sfGFP-His
Plasmid#165479PurposepET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tagDepositorTypeEmpty backboneUseTagsTEV-GFP-6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABCB8 (ABCB8A-c016)
Plasmid#110266PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertABCB8 (ABCB8 Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
HSD17B11
Plasmid#39159PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD17B11 (HSD17B11 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
5WCJ (METTL13)
Plasmid#101270PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMETTL13 (METTL13 Human)
UseBaculovirus expressionTags6xHis-TEVExpressionMutationPromoterpolyhedrinAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CHD1 (CHD1A-c107)
Plasmid#110253PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCHD1 (CHD1 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPb2Cas12a
Plasmid#174686PurposeExpresses 6xHis-MBP tagged Pb2Cas12aDepositorArticleInserthuPb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huLb5Cas12a
Plasmid#174684PurposeExpresses 6xHis-MBP tagged Lb5Cas12aDepositorArticleInserthuLb5Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4258306
Plasmid#154795PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationH. sapiens recode 1PromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits