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FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
HNF4A_P2-226

Plasmid

#31064

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-226 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBullet-pm-n

Plasmid

#53077

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-vac-n

Plasmid

#53075

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Chondromyces

Plasmid

#225078

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Chondromyces)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanI-101

Plasmid

#225065

Purpose

Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanI-101)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2D

Plasmid

#225071

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2D)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts1

Plasmid

#174265

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts2

Plasmid

#174266

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts3

Plasmid

#174267

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_Zeo_TetO_FLAG-AT1R

Plasmid

#222856

Purpose

Mammalian expression plasmid for FLAG-tagged AT1R

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB303_ZFcharm_Kv1_Prnp_SMM

Plasmid

#220841

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Amt LDHA

Plasmid

#209408

Purpose

Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Kaymak et al Cell Metab. 2022 Sep 6;34(9):1298-1311.e

Insert

LdhA shRNA (Ldha Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND6-1397N

Plasmid

#212952

Purpose

Expresses TALED_Left-ND6-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-ND6-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-Cox3.1-1397C-AD.

Plasmid

#212953

Purpose

ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-Cox3.1-1397C-AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Right-ND6-1397C-AD

Plasmid

#212956

Purpose

Expresses TALED_Right-ND6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-ND6-1397C-AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TALED_Left-Cox3.1-1397N.

Plasmid

#212950

Purpose

Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-Cox3.1-1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge