Addgene

Showing: 3841 - 3860 of 5035 results

Results per page:

Sort results by:

SPLICS Mt-ER- Po Long P2A

Plasmid

#164117

Purpose

Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
CMV-NLS-AcrIIA4

Plasmid

#113037

Purpose

Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SV40 NLS-AcrIIA4

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Q769gp120-8his

Plasmid

#100929

Purpose

Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA flag-Msi1

Plasmid

#184029

Purpose

Msi1 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Msi1 (Msi1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PH Akt-Venus

Plasmid

#85223

Purpose

Venus tagged PH domain of Akt for use as a PIP3 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

PH AKT(1-148) (AKT1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE2690

Plasmid

#137717

Purpose

Expresses dimpLbCas12a-SunTag in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dimpLbCas12a

Use

CRISPR

Tags

NLSx3 SV40 and SunTagx10

Expression

Mammalian

Mutation

D156R, G532R, K538V, Y542R, K595R, D832A,

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-RAPH1-F1

Plasmid

#194860

Purpose

Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 1-535

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HCMVgL-FLAG (TB40/E)

Plasmid

#136449

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Use

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBCMV-HaloTag-VPg(FCV)

Plasmid

#167311

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

HaloTag-VPg(FCV)

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA SF-HERC2 F1

Plasmid

#55784

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA Myc-mMEKK2

Plasmid

#44158

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

Mekk2 (Map3k2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
mSIRT3-S

Plasmid

#33310

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA Flag PGC1 T177A S538A

Plasmid

#18093

Depositor

Bruce Spiegelman

Article

Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

Insert

PGC-1a (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

T177A S538A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
Vh-HA-FKBP

Plasmid

#20220

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-ING4

Plasmid

#13289

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING4 (ING4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only