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Plasmid#109947PurposeProtein expression and purification of GNAT2_G-alphaDepositorInsertGNAT2_G-alpha (GNAT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-B-AtAFB2-mCherry
Plasmid#129718PurposeAAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherryDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry
Plasmid#129716PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherryDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6-(S1P-)
Plasmid#32956PurposeFor visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationS1Protease site mutation: R415A/R416APromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ATF6 (1-373)m1
Plasmid#27174PurposeFor mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.DepositorInsertATF6 (ATF6 Human)
UseTagsHAExpressionMammalianMutationContains only aa 1-373 Amino acids 315–317 are c…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (250-536) HA
Plasmid#42209DepositorInsertc-SRC (250-536) HA (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 250-536PromoterCMVAvailable sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
peGFP C2-IST1
Plasmid#218582PurposeExpresses GFP-tagged human IST1 in mammalian cellsDepositorInsertIST1 (IST1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (151-536)
Plasmid#42207DepositorInsertc-SRC (151-536) (SRC Human)
UseTagsExpressionMammalianMutationcontains amino acids 151-536PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (250-536) K298M HA
Plasmid#42210DepositorInsertc-SRC (250-536) HA (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 250-536; K298MPromoterCMVAvailable sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (250-536)
Plasmid#42208DepositorInsertc-SRC (250-536) (SRC Human)
UseTagsExpressionMammalianMutationcontains amino acids 250-536PromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
P119 C-Check
Plasmid#66817PurposeDual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cellsDepositorInserttruncated Enhanced Green Fluorescent Protein and asRED
UseTagsExpressionMammalianMutationPromoterPGK and CMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (WT)
Plasmid#42202DepositorInsertc-SRC (SRC Human)
UseTagsExpressionMammalianMutationL176F mutation compared to reference sequence NM_…PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CMV-Grid2(I677C)
Plasmid#164782Purposecysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligandDepositorInsertGrid2 I677C (Grid2 Mouse)
UseTagsExpressionMammalianMutationI677CPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLC-CUL4A K705R-P2A-Puro
Plasmid#124305PurposeLentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoterDepositorInsertCUL4A (CUL4A Human)
UseLentiviralTagsExpressionMammalianMutationchanged Lysine 705 to ArgininePromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET41s-GST-ATF6a-AD
Plasmid#64774Purposebacterial expression of GST-tagged ATF6 (1-150)DepositorInsertATF6a-AD (1-150) (ATF6 Human)
UseTagsGSTExpressionMammalianMutationC-term deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 315-360, no Cys
Plasmid#180643PurposeBacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.DepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 315-360PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only