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pUT1771-HQ858785
Plasmid#27867DepositorInsertMYB transcription factor from DV027932
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2Δ751-813aa
Plasmid#163603PurposeExpresses BMPR-2 lacking the LIMK-binding region in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe LIMK-binding region (751-813 aa) is deletedPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Rtn4a
Plasmid#86683PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertRtn4a (RTN4 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorInsertbeta actin (ACTB Human)
UseTagsPAGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-Laconic/pCMV-myc-nuc
Plasmid#46307Purposea nuclear-targeted, genetically-encoded sensor for lactateDepositorInsertNLS-Laconic
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 GFP-Rtn4a
Plasmid#86684PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertRtn4a (RTN4 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K
Plasmid#110160PurposeExpression of human eEF2K in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
MO91-dCas9-AID(P182X)
Plasmid#83260PurposeExpresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.DepositorInsertdCas9-AID P182X
UseRetroviralTags3xFLAG-NLS and FLAGExpressionMammalianMutationD10A and H840A in Cas9; P182X in AIDPromoterMSCVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT15547_PEmax-SuperFi-Cas9
Plasmid#184377PurposeMammalian expression of SuperFi-Cas9 PEmax prime editorDepositorInsertPEmax-SuperFi-Cas9
UseCRISPRTagsSV40 NLS and c-myc NLSExpressionMammalianMutationH840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB+BACK)
Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-IFITM3g1 (BB15)
Plasmid#139460PurposeLentiviral vector with gRNA targeting IFITM3; includes puromycin selectable markerDepositorInsertIFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #1
Plasmid#83089PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3L
Plasmid#126588PurposeDNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-dCas9-DNMT3L-NLSExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29 (LM2708)
Plasmid#208965PurposeUnfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast
Plasmid#128010Purposetraffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sitesDepositorInserttraffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
UseDrosophila osc reporter constructTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
DNMT3L-dCas9
Plasmid#126587PurposeDNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-DNMT3L-dCas9-NLSExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB_T2098L-VAMP3-mCherry
Plasmid#92429PurposeExpression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsFKBP rapamycin binding (FRB) domain of mTOR with …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-B103 (LM2907)
Plasmid#208968PurposeUnfused B103 DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
