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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,064 results for: sgRNA

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Showing: 3821 - 3840 of 9064 results

  1. pUC57-Amp_CRISPIE donor B5

    Plasmid
    #172846
    Purpose
    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172846-plasmid-map-sequence-id-340979

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  2. pUC57-Amp_CRISPIE donor B1

    Plasmid
    #172842
    Purpose
    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172842-plasmid-map-sequence-id-340939

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  3. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  4. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  5. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188686-plasmid-map-sequence-id-375116

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Showing: 3821 - 3840 of 9064 results