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pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP

Plasmid

#223214

Purpose

Lentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

Soluble TNFαR (TNFRSF1A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

Plasmid

#224524

Purpose

A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

rox-3xNLS-mCherry-V5-2xSTOP-rox

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 388-606 (OSK domain) (HK75)

Plasmid

#206463

Purpose

Oskar 388-606 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Oskar 388-606 (osk Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Oskar 139-240 (osk Fly)

Use

Tags

PH mcherry

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212830

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

Depositor

Wolfgang Enard

Article

Edenhofer F. et al iScience, 21 June 2024

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Use

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG, TetOn

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212831

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

Depositor

Wolfgang Enard

Article

Edenhofer F. et al iScience, 21 June 2024

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Use

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG, TetOn

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB

Plasmid

#219557

Purpose

PM-targeted iLID-LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-iLID-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211689

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211690

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2

Plasmid

#211691

Purpose

U6-sgRNA-EFS-Cas9-P2A-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210856

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

Plasmid

#182379

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179425

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF alpha, pRSV

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174528

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

Plasmid

#171100

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Neurog2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-571 C-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130118

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130119

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-573 C-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130120

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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