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Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
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pHG4
Plasmid#188252PurposeIntegrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-M
Plasmid#26589DepositorInsertn/a
UseShuttle vector for mycobacteriaTagsExpressionMutationPromoterAvailable sinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJDC182
Plasmid#60854PurposeExpresses firefly luciferaseDepositorInsertFirefly luciferase (optimized for Mycobacteria)
UseTagsExpressionBacterialMutationcodon optimized for MycobacteriaPromoterhsp60Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sen1
Plasmid#99994PurposeShuttle yeast Sen1 into Gateway destination vectorDepositorInsertSen1 (SEN1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBUN276
Plasmid#60673Purposeoverexpression of M. tuberculosis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTags6 codons of M. bovis BCG hsp60ExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM0494
Plasmid#128981PurposeLEU2 preassembled entry vectorDepositorInsertLEU2 5' homology-ConLS-sfGFP dropout-ConR1-LEU2 3' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Cka2
Plasmid#99980PurposeShuttle yeast Cka2 into Gateway destination vectorDepositorInsertCka2 (CKA2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Utp16
Plasmid#99985PurposeShuttle yeast Utp16 into Gateway destination vectorDepositorInsertUtp16 (BUD21 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p006-Banana-Late
Plasmid#112251PurposeATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.DepositorInsertATF1 under osmY promoter
UseTagsExpressionBacterialMutationPromoterosmYAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST5552
Plasmid#36255PurposeMycobacteria replicating vector expressing eGFP.DepositorInserteGFP
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModifiedHsp60Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pST-KiT
Plasmid#44562Purposefor integrative inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-G418-C4
Plasmid#160342PurposeHigh-copy expression vector for K. marxianus, G418 selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-G418
Plasmid#160012PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+Lux
Plasmid#26160DepositorInsertG13 promoter + Bacterial luciferase operon
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only