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PCMV-intron myc Rab2 S20N

Plasmid

#46780

Purpose

expression of S20N Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S20N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
CMV-CRY2oligo-mcherry-ANXA11(R235Q)

Plasmid

#164205

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R235Q

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV) GFP (w513-1)

Plasmid

#19170

Purpose

Gateway entry vector for miRNA expression under CMV promoter and GFP reporter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂304)

Plasmid

#24810

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-304.

Promoter

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
p2T-CMV-miniSdd7-BE4max-BlastR

Plasmid

#204851

Purpose

For cytosine base editing using miniSdd7 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab11 S25N

Plasmid

#46786

Purpose

expression of S25N Rab11a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB11A S25N (RAB11A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S25N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ (106∂)

Plasmid

#24814

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 1-106

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3C-GFP

Plasmid

#123110

Purpose

Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C64S,C65S-FLAG

Plasmid

#198186

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 64/65 mutates t…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV Renilla Luciferase L5-L2

Plasmid

#62186

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Renilla luciferase

Use

Luciferase; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pVQ CMV NanoV1Mutant-2a-EGFP ferritin

Plasmid

#79650

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Article

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanilloid 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

isoleucine 679 changed to lysine

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-sport2-mGCN5 FU, FLAG Tagged

Plasmid

#23098

Depositor

Sharon Dent

Article

Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

Insert

mouse Gcn5 mRNA (Kat2a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

missing 50 bp upstream of ATG start site.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3B-copGFP-Puro

Plasmid

#107342

Purpose

Expresses UBE3B fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

Ubiquitin-protein ligase E3B (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E

Plasmid

#100147

Purpose

Expresses EIF3E

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3E (EIF3E Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

Plasmid

#194015

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.1)
eGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV and u6

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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