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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

Plasmid

#163179

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285

Plasmid

#31060

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Ovtm

Plasmid

#51888

Purpose

expression vectore in mammalian cells

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mFbxl3 (Fbxl3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

ovtm (I364T)

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
CDK16_HUMAN_D0

Plasmid

#79696

Purpose

This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDK16 (CDK16 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
MERTK_HUMAN_D0

Plasmid

#79705

Purpose

This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MERTK (MERTK Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

Plasmid

#163178

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-M-C4

Plasmid

#73427

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor C4 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive wild-type S. pyogenes promoter

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pαH-S-HRV3C/D614G

Plasmid

#164568

Purpose

SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-HRV3C-D614G variant)

Use

Tags

2X Strep-Tag II and 8X His tag

Expression

Mammalian

Mutation

D614G

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

Plasmid

#163138

Purpose

Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

Plasmid

#91882

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pdCas9-M-3F2

Plasmid

#73428

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor C4 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive wild-type S. pyogenes promoter

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CHK2_HUMAN_D0

Plasmid

#79715

Purpose

This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CHK2 (CHEK2 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86023

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Pardis Sabeti, Jeremy Luban

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed threonine 230 to…

Promoter

CMV IE

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Pardis Sabeti, Jeremy Luban

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DAPK1_HUMAN_D0

Plasmid

#79728

Purpose

This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK1 (DAPK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge